{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T16:22:28Z","timestamp":1783441348708,"version":"3.54.6"},"reference-count":62,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess different lots and brands of sterile preservative\u2010free dexamethasone sodium phosphate (dexamethasone SP) for evidence of bacterial contamination via culture\u2010dependent and independent methods, and quantify endotoxin. Broadly, the objective is to further investigate potential causes for fibrin web (FW) development after canine cataract surgery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Procedures<\/jats:title>\n                    <jats:p>Five samples from two lots of three brands of dexamethasone SP (30 samples total) and reagent controls were collected. All samples underwent conventional aerobic and capnophilic bacterial culture, as well as kinetic chromogenic endotoxin quantification. Deoxyribonucleic acid (DNA) extraction, 16S ribosomal ribonucleic acid (rRNA) sequencing, sequence analysis, and classification were performed on all samples. Statistical analysis assessed DNA concentration, \u03b1 and \u03b2 diversity, and sample biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bacterial cultures were positive in 0\/10 Auromedics, 1\/10 Fresenius Kabi, and 4\/10 Somerset Pharma LLC dexamethasone SP samples. The bacteria identified (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                      <\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                      <\/jats:styled-content>\n                      , and\n                      <jats:italic>Staphylococcus oralis<\/jats:italic>\n                      ) were likely present due to contaminants during plating. Endotoxin quantification was below the limit of detection of the assay (0.5 EU\/mL). There were no statistically significant differences in \u03b1 or \u03b2 diversity between brands. Differential abundance testing found\n                      <jats:italic>Lactococcus<\/jats:italic>\n                      and\n                      <jats:italic>Acetitomaculum<\/jats:italic>\n                      as genus\u2010level biomarkers for the Auromedics brand and\n                      <jats:italic>Solobacterium<\/jats:italic>\n                      for the Somerset brand of dexamethasone SP. The most common bacterial families recovered were\n                      <jats:italic>Bacillaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Clostridiaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Enterobacteriaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Lachnospiraceae<\/jats:italic>\n                      ,\n                      <jats:italic>Lactobacillaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Muribaculaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Propionilbacteriaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Staphylococcaeae<\/jats:italic>\n                      , and\n                      <jats:italic>Xanthobacteraceae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>There were no significant dexamethasone SP brand\u2010specific differences. Endotoxin was below the limit of detection of the assay.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vop.70186","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T07:16:14Z","timestamp":1781507774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial Communities Identified by Standard Culture and\n                    <scp>16S rRNA<\/scp>\n                    Amplicon Sequencing in Ophthalmic Preservative\u2010Free Dexamethasone\n                    <scp>SP<\/scp>\n                    Used in Canine Cataract Surgery"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1759-3896","authenticated-orcid":false,"given":"Aaron C.","family":"Sieve","sequence":"first","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9874-8935","authenticated-orcid":false,"given":"Elizabeth A.","family":"Giuliano","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2883-507X","authenticated-orcid":false,"given":"Zachary L.","family":"McAdams","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8146-0580","authenticated-orcid":false,"given":"Aida","family":"Vient\u00f3s\u2010Plotts","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3053-7269","authenticated-orcid":false,"given":"Aaron C.","family":"Ericsson","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(97)50107-0"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2005.00352.x"},{"key":"e_1_2_13_4_1","first-page":"283","article-title":"Cataracts in 44 Dogs (77 Eyes): A Comparison of Outcomes for no Treatment, Topical Medical Management, or Phacoemulsification With Intraocular Lens Implantation","volume":"52","author":"Lim C. 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