{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:09:09Z","timestamp":1788944949299,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess the clinical effects of a compounded 0.03% tacrolimus in 0.75% cross\u2010linked hyaluronic acid (XHA) on tear quantity, quality, and ocular surface health in dogs with evaporative dry eye disease (EDED).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Procedures<\/jats:title>\n                    <jats:p>Sixteen client\u2010owned dogs diagnosed with EDED (normal aqueous production yet reduced stability) were enrolled in this prospective, open\u2010label clinical trial. Patients were treated with the compounded formulation in affected eyes twice daily for 45\u2009days. Ophthalmic assessments, including Schirmer tear test\u20101 (STT\u20101), tear film breakup time (TFBUT), punctate fluorescein staining (PFS), lissamine green staining, and conjunctival hyperemia scoring, were performed during designated morning appointments at Day 0, Day 15, and Day 45. Data were analyzed using repeated\u2010measures ANOVA and post hoc tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      STT\u20101 values increased significantly from 18.7\u2009\u00b1\u20092.3\u2009mm\/min at baseline to 21.7\u2009\u00b1\u20094.1\u2009mm\/min at Day 45 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.004). Tear film stability improved markedly, with TFBUT rising from 3.3\u2009\u00b1\u20090.7\u2009s to 5.2\u2009\u00b1\u20091.4\u2009s (Day 15) and 8.1\u2009\u00b1\u20092.4\u2009s (Day 45) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Ocular surface health improved, evidenced by PFS scores decreasing from 1.8\u2009\u00b1\u20090.9 to 0.3\u2009\u00b1\u20090.4 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and lissamine green scores dropping from 1.9\u2009\u00b1\u20091.1 to 0.1\u2009\u00b1\u20090.3 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Conjunctival hyperemia scores declined significantly by Day 45 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.041).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Twice\u2010daily administration of compounded XHA\/tacrolimus resulted in significant improvements in tear quantity, stability, and corneoconjunctival integrity. Combining immunomodulation with long\u2010acting lubrication in a single formulation offers a promising, practical therapeutic strategy for efficiently managing canine qualitative dry eye disease in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vop.70251","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T04:15:37Z","timestamp":1787372137000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Compounded 0.03% Tacrolimus in Cross\u2010Linked Hyaluronate Improves Ocular Surface Health in Dogs With Evaporative Dry Eye Disease"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0103-0127","authenticated-orcid":false,"given":"Lionel","family":"Sebbag","sequence":"first","affiliation":[{"name":"Koret School of Veterinary Medicine The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7289-5850","authenticated-orcid":false,"given":"Eunice Q. A.","family":"Neta","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Zootechny Federal University of Bahia, UFBA  Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catharina R.","family":"de Farias","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Zootechny Federal University of Bahia, UFBA  Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3574-3723","authenticated-orcid":false,"given":"Ana Cl\u00e1udia S.","family":"Raposo","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Zootechny Federal University of Bahia, UFBA  Salvador Brazil"},{"name":"Council for Scientific and Technological Development\u2013CNPq  Brasilia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5093-6841","authenticated-orcid":false,"given":"Arianne P.","family":"Ori\u00e1","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Zootechny Federal University of Bahia, UFBA  Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.08.003"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13066"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.12968\/coan.2019.24.5.240"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1989.01070020276038"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748\u20105827.2006.00185.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.13382"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.70042"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259\u2010025\u201010967\u20107"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13022"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010019\u20102203\u20103"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/vetr.1682"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13326"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13293"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0195\u20105616(90)50071\u20106"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13044"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2003.12.009"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.12968\/coan.2019.24.6.319"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2017.272"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16\u201020848"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099766"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/460437"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13620\u2010023\u201000259\u20104"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1415658"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259\u2010024\u201010559\u2010x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.70013"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463\u20105224.2005.00390.x"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2020.909.416"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12634"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.5935\/0004\u20102749.20180059"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103\u201084782008000200048"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12524"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12762"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/487592"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748\u20105827.2011.01024.x"},{"key":"e_1_2_11_36_1","unstructured":"C. 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E.Carbia \u201cEvaluation of Topically Applied Cross\u2010Linked Hyaluronic Acid (Remend\u00ae) on the Ocular Surface of Clinically Healthy Dogs \u201dACVO 2022 Conference Proceedings (Palm Springs CA October 26\u201329 2022) 130."}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vop.70251","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:45:51Z","timestamp":1788943551000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.70251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/vop.70251"],"URL":"https:\/\/doi.org\/10.1111\/vop.70251","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]},"assertion":[{"value":"2026-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70251"}}