{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:43:29Z","timestamp":1792212209119,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T320OD011130"],"award-info":[{"award-number":["T320OD011130"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To evaluate the effect of flunixin meglumine, a systemic non\u2010steroidal anti\u2010inflammatory drug (NSAID), on the antiproteases, A2M and A1AT, in equine plasma and PRF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A prospective cross\u2010sectional study. Plasma, solid PRF, and liquid PRF were collected from a control\u2010group that included healthy horses not treated with NSAIDs within the last 3\u2009months. The NSAID\u2010group were ophthalmology patients with no systemic inflammatory disease, and all received a full dose of flunixin meglumine (1.1\u2009mg\/kg BID PO or IV) for three or more days prior to collection. Samples were collected and prepared using a standardized protocol for plasma and PRF preparation. Concentrations of A2M and A1AT were analyzed with ELISA kits. Statistical analysis with Mann\u2013Whitney\n                      <jats:italic>U<\/jats:italic>\n                      tests and Wilcoxon signed\u2010rank tests was performed using significance set at\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      No significant differences were found between the control\u2010group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912) and NSAID\u2010group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912) for any of the blood products (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Plasma had a significantly higher amount of A2M compared to solid and liquid PRF (both\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Solid PRF had the highest concentration of A1AT and was significantly higher when compared to liquid PRF (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), but not when compared to plasma (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.071).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The systemic NSAID, flunixin meglumine, does not appear to significantly alter antiprotease concentrations in equine plasma or PRF. Although PRF should not be used as a replacement for plasma, solid PRF may be a good adjunctive therapy for the treatment of keratomalacia due to its high A1AT concentration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vop.70280","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:30:07Z","timestamp":1792211407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Impact of Systemic Treatment With Flunixin Meglumine on the Two Antiproteases Alpha\u20102\u2010Macroglobulin (\n                    <scp>A2M<\/scp>\n                    ) and Alpha\u20101\u2010Antitrypsin (\n                    <scp>A1AT<\/scp>\n                    ) in Equine Plasma and Platelet\u2010Rich Fibrin (\n                    <scp>PRF<\/scp>\n                    )"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4909-5599","authenticated-orcid":false,"given":"Kaitlyn R.","family":"Scheunemann","sequence":"first","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9163-214X","authenticated-orcid":false,"given":"Katrina E. V.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8238-5455","authenticated-orcid":false,"given":"Kimberly","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongben","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biobehavioral Laboratory, School of Nursing University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0176-4240","authenticated-orcid":false,"given":"Cody","family":"McGrath","sequence":"additional","affiliation":[{"name":"Biobehavioral Laboratory, School of Nursing University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5912-9525","authenticated-orcid":false,"given":"Kimberly R.","family":"Love","sequence":"additional","affiliation":[{"name":"KR Love Quantitative Consulting and Collaboration  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-7790","authenticated-orcid":false,"given":"Michala","family":"de Linde Henriksen","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2004.04.007"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463\u20105224.2007.00546.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463\u20105224.2000.00093.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctep.2005.03.012"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2003.12.005"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463\u20105224.2004.04052.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2003.64.1081"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.17.2.174.5602"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1342345"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787\u2010021\u201000820\u2010z"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.25.03.0204"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci8100213"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40729\u2010016\u20100052\u20104"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201048933\u20105"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13148"},{"key":"e_1_2_10_17_1","volume-title":"Horse Alpha 2 Macroglobulin (A2MG) ELISA Kit Product Manual, Version 17.0. Catalog #MBS014509","author":"MyBioSource","year":"2026"},{"key":"e_1_2_10_18_1","volume-title":"AffiELISA Equine A1AT ELISA Kit\u20102 Plates Product Data Sheet. Catalog #AFG\u2010GNS\u20101640","author":"AffiGEN","year":"2026"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","unstructured":"M.Herv\u00e9 RVAideMemoire: Testing and Plotting Procedures for Biostatistics. R Package Version 0.9\u201083\u201012 https:\/\/doi.org\/10.32614\/CRAN.package.RVAideMemoire. (Accessed Oct 14th 2026).","DOI":"10.32614\/CRAN.package.RVAideMemoire"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcm.2016.02.012"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1998.0637"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374980120011301"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.70057"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1330709"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2023.06.004"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.77.6.627"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12125"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12187"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1417842"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.1999.0407"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042\u20103306.1982.tb02443.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1335972"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.24.11.0363"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2003.12.003"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70280","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vop.70280","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:30:11Z","timestamp":1792211411000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.70280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,16]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1111\/vop.70280"],"URL":"https:\/\/doi.org\/10.1111\/vop.70280","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,16]]},"assertion":[{"value":"2026-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70280"}}