{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:15:00Z","timestamp":1783466100918,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,2,9]],"date-time":"2013-02-09T00:00:00Z","timestamp":1360368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Background and Objectives<\/jats:title>A number of DNA<\/jats:styled-content>\u2010based methods to genotype the alleles coding for HNA<\/jats:styled-content> have been described, but all require the separate amplification and analysis of each allele. The aim was to develop a DNA<\/jats:styled-content>\u2010based method for simultaneous detection of HNA<\/jats:styled-content>\u20101, HNA<\/jats:styled-content>\u20103, HNA<\/jats:styled-content>\u20104 and HNA<\/jats:styled-content>\u20105 alleles.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>An allele\u2010specific primer extension method was used in combination with magnetic beads from L<\/jats:styled-content>uminex technology. PCR<\/jats:styled-content>\u2013sequence\u2010specific primers (SSP<\/jats:styled-content>) was used to resolve the presence of the HNA<\/jats:styled-content>\u20101b allele in samples assigned by the Luminex bead assay as HNA<\/jats:styled-content>\u20101a\/\u20101b\/\u20101c or HNA<\/jats:styled-content>\u20101b\/\u20101c. HNA<\/jats:styled-content> allele frequencies were determined in a panel of 140 randomly selected English Caucasoid blood donors.<\/jats:p><\/jats:sec>Results<\/jats:title>HNA<\/jats:styled-content> allelic types were compared with historical results, and 100% concordance was found. Only eight of the 97 samples used in the validation required additional testing by PCR<\/jats:styled-content>\u2013SSP<\/jats:styled-content>. Allele frequencies were determined in the blood donor population as follows: 0\u00b7318 for HNA<\/jats:styled-content>\u20101a, 0\u00b7668 for HNA<\/jats:styled-content>\u20101b, 0\u00b7014 for HNA<\/jats:styled-content>\u20101c, 0\u00b7768 for HNA<\/jats:styled-content>\u20103a, 0\u00b7232 for HNA<\/jats:styled-content>\u20103b, 0\u00b7882 for HNA<\/jats:styled-content>\u20104a, 0\u00b7118 for HNA<\/jats:styled-content>\u20104b, 0\u00b7736 for HNA<\/jats:styled-content>\u20105a and 0\u00b7264 for HNA<\/jats:styled-content>\u20105b.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>A multiplex Luminex bead assay for the simultaneous detection of HNA<\/jats:styled-content>\u20101, HNA<\/jats:styled-content>\u20103, HNA<\/jats:styled-content>\u20104 and HNA<\/jats:styled-content>\u20105 alleles is described that enables rapid typing of donors to support HNA<\/jats:styled-content> alloimmunized patients who require HNA<\/jats:styled-content>\u2010compatible blood products.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12016","type":"journal-article","created":{"date-parts":[[2013,2,9]],"date-time":"2013-02-09T11:08:40Z","timestamp":1360408120000},"page":"65-72","source":"Crossref","is-referenced-by-count":27,"title":["Determination of human neutrophil antigen\u20101, \u20103, \u20104 and \u20105 allele frequencies in E<\/scp>nglish C<\/scp>aucasoid blood donors using a multiplex fluorescent DNA<\/scp>\u2010based assay"],"prefix":"10.1111","volume":"105","author":[{"given":"S. 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