{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:46:17Z","timestamp":1783003577106,"version":"3.54.5"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T00:00:00Z","timestamp":1413849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Umbilical cord blood (<jats:styled-content style=\"fixed-case\">UCB<\/jats:styled-content>) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell (<jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>s) subtypes were viable and functional until 96\u00a0h after collection, even stored at room temperature. Now, we analyzed the viability and functionality of the cells before and after cryopreservation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Twenty <jats:styled-content style=\"fixed-case\">UCB<\/jats:styled-content> units were analyzed at 24 and 96\u00a0h after collection, frozen for 6\u00a0months, thawed and re\u2010evaluated. <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>s were analyzed by flow cytometry, viability by 7\u2010<jats:styled-content style=\"fixed-case\">AAD<\/jats:styled-content> and clonogenic assays (<jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>) were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 96\u00a0h of storage, no substantial loss of <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content> was found (median 7\u00b7320\u00a0\u00d7\u00a010<jats:sup>6\u00a0<\/jats:sup>\u00d7\u00a06\u00b7305\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>). Percentage and viability <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> cells, B\u2010cell precursors and mesenchymal stem cells were not affected. However, mature B and T lymphocytes as well as granulocytes had a substantial loss. <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content> growth was observed in all samples. Prefreezing storage of 96\u00a0h was associated with a relative loss of colony formation (median 12%). Postthaw, this loss had a median of 49% (24\u00a0h samples) to 56% (96\u00a0h samples).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The delay of 96\u00a0h before <jats:styled-content style=\"fixed-case\">UCB<\/jats:styled-content> processing is possible, without a prohibitive impairment of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> loss in number and functionality.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12199","type":"journal-article","created":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T11:19:41Z","timestamp":1413890381000},"page":"72-81","source":"Crossref","is-referenced-by-count":9,"title":["Umbilical cord blood <scp>CD<\/scp>34<sup>+<\/sup> stem cells and other mononuclear cell subtypes processed up to 96\u00a0h from collection and stored at room temperature maintain a satisfactory functionality for cell therapy"],"prefix":"10.1111","volume":"108","author":[{"given":"F. G.","family":"Pereira\u2010Cunha","sequence":"first","affiliation":[{"name":"Flow Cytometry Laboratory Haematology Hemotherapy Center University of Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S. S.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Public Umbilical Cord Blood Bank Haematology Hemotherapy Center University of Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. C.","family":"Reis\u2010Alves","sequence":"additional","affiliation":[{"name":"Cryopreservation Laboratory Haematology Hemotherapy Center University of Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. T.","family":"Olalla Saad","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Medical Sciences University of Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Metze","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medical Sciences University of Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Lorand\u2010Metze","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Medical Sciences University of Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2. C. M.","family":"Luzo","sequence":"additional","affiliation":[{"name":"Public Umbilical Cord Blood Bank Haematology Hemotherapy Center University of Campinas Campinas 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