{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T20:15:36Z","timestamp":1767989736961,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T00:00:00Z","timestamp":1413849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Extracorporeal photochemotherapy (<jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content>) is an established therapy in various diseases, such as cutaneous T\u2010cell lymphoma and graft\u2010versus\u2010host disease. This study was performed to investigate the practicability of a flow cytometric T\u2010cell evaluation after <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> as a tool to validate the quality of <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> procedures and to enable the comparability of treatments with different <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> devices.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Peripheral blood mononuclear cells (<jats:styled-content style=\"fixed-case\">PBMNC<\/jats:styled-content>s) of healthy volunteer blood donors were treated by offline <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content>. To quantify the effect of <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> on T cells <jats:italic>in vitro<\/jats:italic>, phosphatidylserine exposure and 7\u2010aminoactinomycin D (7\u2010<jats:styled-content style=\"fixed-case\">AAD<\/jats:styled-content>) reactivity as well as the proliferative activity of phytohaemagglutinin\u2010induced, viable <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup> lymphocytes were analysed by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expected T\u2010cell death after <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> was confirmed by 7\u2010<jats:styled-content style=\"fixed-case\">AAD<\/jats:styled-content> measurements. Phosphatidylserine exposure gradually increased between 20 and 70\u00a0h after <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content>. Treatment\u2010related inhibition of T\u2010cell proliferation was 92\u00b76\u00a0\u00b1\u00a01\u00b74%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The combination of viability, phosphatidylserine exposure and T\u2010cell division analyses by flow cytometry in a single\u2010platform system provides a valuable tool to validate <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> procedures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12200","type":"journal-article","created":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T11:19:24Z","timestamp":1413890364000},"page":"82-88","source":"Crossref","is-referenced-by-count":14,"title":["T\u2010Cell death, phosphatidylserine exposure and reduced proliferation rate to validate extracorporeal photochemotherapy"],"prefix":"10.1111","volume":"108","author":[{"given":"D.","family":"Schmid","sequence":"first","affiliation":[{"name":"Blood Group Serology and Transfusion Medicine Salzburg University Hospital (SALK) Salzburg Austria"},{"name":"Spinal Cord Injury &amp; Tissue Regeneration Center Salzburg (SCI\u2010TReCS) Paracelsus Medical University (PMU) Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Grabmer","sequence":"additional","affiliation":[{"name":"Blood Group Serology and Transfusion Medicine Salzburg University Hospital (SALK) Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Streif","sequence":"additional","affiliation":[{"name":"Blood Group Serology and Transfusion Medicine Salzburg University Hospital (SALK) Salzburg Austria"},{"name":"Spinal Cord Injury &amp; Tissue Regeneration Center Salzburg (SCI\u2010TReCS) Paracelsus Medical University (PMU) Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Lener","sequence":"additional","affiliation":[{"name":"Blood Group Serology and Transfusion Medicine Salzburg University Hospital (SALK) Salzburg Austria"},{"name":"Spinal Cord Injury &amp; Tissue Regeneration Center Salzburg (SCI\u2010TReCS) Paracelsus Medical University (PMU) Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Schallmoser","sequence":"additional","affiliation":[{"name":"Blood Group Serology and Transfusion Medicine Salzburg University Hospital (SALK) Salzburg Austria"},{"name":"Spinal Cord Injury &amp; 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