{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T16:20:40Z","timestamp":1779294040085,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T00:00:00Z","timestamp":1417651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cytochrome P450 1A2 (CYP1A2) is a cytochrome enzyme with a pivotal role in hepatic drug metabolism. Data from<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2<jats:sup>(\u2212\/\u2212)<\/jats:sup>mouse suggest that<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 plays a role in aspects of hepatic iron toxicity. The aim of this study was to assess the activity of<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 in relation to hepatic iron load in patients with transfusion\u2010dependent \u03b2\u2010thalassaemia major.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The<jats:sup>13<\/jats:sup>C\u2010methacetin continuous breath test was performed on 30 consecutive patients with transfusion\u2010dependent \u03b2\u2010thalassaemia major.<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 activity was measured by the rate at which the<jats:sup>13<\/jats:sup>C substrate is metabolized and exhaled expressed as percentage dose recovery (<jats:styled-content style=\"fixed-case\">PDR<\/jats:styled-content>) per hour.<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 activity was correlated with clinical and laboratory parameters and hepatic iron accumulation by T2* magnetic resonance imaging (T2*<jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cytochrome P450 1A2 activity in patients with transfusion\u2010dependent \u03b2\u2010 thalassaemia major was positivity correlated with plasma ferritin levels. No correlation was found with age, duration and amount of red blood cell transfusion and type of iron chelation therapy. Low<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 activity was negatively associated with hepatic iron accumulation (T2*<jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content>\u00a0\u2264\u00a06\u00b73\u00a0ms); adjusted odds ratio (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>; 95%<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>) for hepatic iron accumulation in patients with low<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 activity was 0\u00b7047 (0\u00b7003\u20130\u00b772;<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7021). Of the six patients with decreased activity of<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2, five had no hepatic iron accumulation and one had mild hepatic iron accumulation by T2*<jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Activity of<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 is associated with hepatic iron accumulation in patients with transfusion\u2010depended \u03b2\u2010thalassaemia major. Further studies are needed to assess the exact role of<jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>1A2 in iron metabolism in human.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12223","type":"journal-article","created":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T14:06:45Z","timestamp":1417788405000},"page":"268-273","source":"Crossref","is-referenced-by-count":2,"title":["Activity of cytochrome P450 1A2 in relation to hepatic iron accumulation in transfusion\u2010dependent \u03b2\u2010thalassaemia major patients"],"prefix":"10.1111","volume":"108","author":[{"given":"E.","family":"Shteyer","sequence":"first","affiliation":[{"name":"Pediatric Gastroenterology Unit Hadassah\u2010Hebrew University Medical Center Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Nitzan","sequence":"additional","affiliation":[{"name":"Pediatric Gastroenterology Unit Hadassah\u2010Hebrew University Medical Center Jerusalem 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