{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T07:45:23Z","timestamp":1696232723765},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T00:00:00Z","timestamp":1434672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>At Japanese Red Cross (<jats:styled-content style=\"fixed-case\">JRC<\/jats:styled-content>) Blood Centers, all donated blood is screened for hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) by serological and nucleic acid amplification testing. Donor plasma that tested reactive for anti\u2010<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> by serological test is disqualified even if the donor tests negative for <jats:styled-content style=\"fixed-case\">HCV RNA<\/jats:styled-content>. These test results reflect both true\u2010positive results because of past <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> infection and false\u2010positive results because the cross\u2010reactivity of plasma IgG, which current testing methods are unable to distinguish. To characterize these antibody test results, we examined the neutralizing activity of these plasma samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Donor plasma samples that tested reactive for anti\u2010<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> by serological test but negative for <jats:styled-content style=\"fixed-case\">HCV RNA<\/jats:styled-content> (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>43) were analysed for determining their neutralizing activities measured by the inhibition of the cellular entry of pseudoparticles harbouring <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> envelope glycoproteins (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>pp).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Strong and broad neutralizing activities against <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>pp entry similar to the samples that tested reactive for anti\u2010<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> serological test and positive for <jats:styled-content style=\"fixed-case\">HCV RNA<\/jats:styled-content> (considered to be derived from individuals with chronic <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> infection) were observed in three of 43 plasma samples from donors who tested anti\u2010<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> reactive but <jats:styled-content style=\"fixed-case\">HCV RNA<\/jats:styled-content> negative.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>By examining the neutralizing activities of plasma samples, we identified individuals with a past <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> infection from those in whom we were unable to confirm <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> infection according to the current testing algorithms of <jats:styled-content style=\"fixed-case\">JRC<\/jats:styled-content>, which do not perform anti\u2010<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> confirmatory tests.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12301","type":"journal-article","created":{"date-parts":[[2015,6,20]],"date-time":"2015-06-20T08:04:37Z","timestamp":1434787477000},"page":"319-326","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of neutralization profiles for anti\u2010<scp>HCV<\/scp> reactive donor samples with or without detectable <scp>HCV RNA<\/scp>"],"prefix":"10.1111","volume":"109","author":[{"given":"A.","family":"Kuroishi","sequence":"first","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Center Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yasui","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Center Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Matsukura","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Center Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Tani","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Center Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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