{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T16:57:17Z","timestamp":1758905837804,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T00:00:00Z","timestamp":1434499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012636","name":"NYSTEM IDEA","doi-asserted-by":"crossref","award":["C026431"],"award-info":[{"award-number":["C026431"]}],"id":[{"id":"10.13039\/100012636","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Epigenetic modifications tightly regulate the gene expression and cellular function of haematopoietic stem cells. Histone deacetylase inhibitors (<jats:styled-content style=\"fixed-case\">HDACI<\/jats:styled-content>s) alter the gene expression profile of cord blood (<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> cells by controlling the genes involved in chromatin modification, thereby influencing the self\u2010renewal, maintenance and expansion of haematopoietic stem and progenitor cells (<jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The class I and <jats:styled-content style=\"fixed-case\">II HDACI<\/jats:styled-content>s, valproic acid and scriptaid, were utilized to expand <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> cells ex\u00a0vivo. The gene profiling was performed on <jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content> using Illumina microarray, Gene<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> MetaCore<jats:sup>\u2122<\/jats:sup> and Ingenuity pathway analyses. The molecular analyses were performed using Q\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and Western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Each <jats:styled-content style=\"fixed-case\">HDACI<\/jats:styled-content> treatment of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> cells created unique epigenetic and molecular signatures that governed chromatin modification required for cellular and functional behaviour of stem cells. Gene<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> MetaCore<jats:sup>\u2122<\/jats:sup> and Ingenuity pathway analyses established the molecular understanding of epigenetically regulated <jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content>s in the presence of scriptaid and <jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content> that revealed different network(s) of potential regulators during erythropoiesis. <jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content> induced transcriptional activation of the glucocorticoid receptor (<jats:styled-content style=\"fixed-case\">GCR<\/jats:styled-content>) and an increase in the intracellular signalling of signal transducers and activators of transcription (<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>) required during stress erythropoiesis. Canonical Wnt signalling and many epigenetically regulated chromatin remodellers were significantly influenced so as to establish maintenance and regulation of <jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Treatment with Individual <jats:styled-content style=\"fixed-case\">HDACI<\/jats:styled-content>s has demonstrated significantly unique epigenetic and molecular signatures of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content>. This study identifies potential key regulators of <jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content> and gives insights into the clinically important processes of <jats:styled-content style=\"fixed-case\">HSPC<\/jats:styled-content> expansion and haematopoietic lineage development for transplantation purposes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12303","type":"journal-article","created":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T18:40:23Z","timestamp":1434566423000},"page":"79-89","source":"Crossref","is-referenced-by-count":4,"title":["Epigenetic and molecular signatures of cord blood <scp>CD<\/scp>34<sup>+<\/sup> cells treated with histone deacetylase inhibitors"],"prefix":"10.1111","volume":"110","author":[{"given":"D.","family":"Gajzer","sequence":"first","affiliation":[{"name":"Division of Hematology\/Medical Oncology Department of Medicine Tisch Cancer Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ross","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology Department of Medicine Tisch Cancer Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Winder","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology Department of Medicine Tisch Cancer Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Navada","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology Department of Medicine Tisch Cancer Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medicine Bioinformatics Core Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Silverman","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology Department of Medicine Tisch Cancer Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology Department of Medicine Tisch Cancer Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.10.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-314567"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI70313"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3063"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-38404-2_3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1190614"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-02-140806"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.21141"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2013.12.562"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-05-424846"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1868-7083-4-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/895343"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","unstructured":"HsuJ HuangH\u2010T LeeC\u2010T et\u00a0al.:Chromatin Remodeling Enzyme CHD7 Negatively Regulate Hematopoietic Stem Cell Function [abstract]. 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