{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:23:33Z","timestamp":1791296613785,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>At Canadian Blood Services, buffy coat (<jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>) platelet concentrates (<jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s) show a generally lower bacterial contamination rate than apheresis <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s. This study investigated whether the <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> production method contributes to this observation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Whole blood (<jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>) inoculated with eight bacterial strains was processed using the <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content> method. Bacteria were enumerated throughout <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> production and subsequent <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> storage. Endotoxin production and bacterial adhesion to <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> bags were evaluated during <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> storage. <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> quality was monitored by <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>62P expression (flow cytometry) and changes in dynamic light scattering (Thrombo<jats:styled-content style=\"fixed-case\">LUX<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>During overnight <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> hold, <jats:italic>Staphylococcus epidermidis<\/jats:italic> titres remained unchanged, commercial <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Klebsiella pneumoniae<\/jats:italic> were eliminated and the remaining organisms proliferated to high concentrations. Through <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> production, bacteria segregated preferentially towards the cellular fractions compared to plasma (<jats:italic>P<\/jats:italic> &lt; 0\u00b705). During <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> storage, most bacteria adhered to the <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> bags and Gram negatives produced clinically significant endotoxin levels. Changes in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>62P expression or Thrombo<jats:styled-content style=\"fixed-case\">LUX<\/jats:styled-content> scoring did not consistently reflect bacterial contamination in <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> hold during <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> production does not have a broad\u2010spectrum bactericidal effect, and therefore, other factors contribute to low rates of contamination in <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12427","type":"journal-article","created":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T07:57:32Z","timestamp":1468915052000},"page":"333-340","source":"Crossref","is-referenced-by-count":21,"title":["Bacterial survival and distribution during buffy coat platelet production"],"prefix":"10.1111","volume":"111","author":[{"given":"M.","family":"Taha","sequence":"first","affiliation":[{"name":"Canadian Blood Services Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kalab","sequence":"additional","affiliation":[{"name":"Agriculture and Agri\u2010Food Canada Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.\u2010L.","family":"Yi","sequence":"additional","affiliation":[{"name":"Canadian Blood Services Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Maurer","sequence":"additional","affiliation":[{"name":"LightIntegra Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Canadian Blood Services Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Schubert","sequence":"additional","affiliation":[{"name":"Canadian Blood Services Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ramirez\u2010Arcos","sequence":"additional","affiliation":[{"name":"Canadian Blood Services Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-655944"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13154"},{"key":"e_1_2_6_4_1","unstructured":"Fatalities Reported to FDA Following Blood Collection and Transfusion. 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