{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T18:56:05Z","timestamp":1788116165850,"version":"build-2803163510"},"reference-count":11,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T00:00:00Z","timestamp":1502668800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The Spectra Optia<jats:sup>\u00ae<\/jats:sup> continuous mononuclear cell (<jats:styled-content style=\"fixed-case\">CMNC<\/jats:styled-content> apheresis) system has emerged as the preferred device in peripheral blood stem cell collections over the original two\u2010step Spectra Optia<jats:sup>\u00ae<\/jats:sup> mononuclear cell (<jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content> apheresis) system. Until now, no comparative data were available for non\u2010stimulated <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content> collections that are required for immunotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We compared collection parameters and product composition for Spectra Optia <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>\u2010 as well as <jats:styled-content style=\"fixed-case\">CMNC<\/jats:styled-content>\u2010apheresis systems in non\u2010stimulated <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content> collections from 35 registry donors intended for donor lymphocyte infusions. In a subsequent analysis, different centrifugation forces (determined as packing factor or <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>) were investigated regarding target cell yield and contamination in 61 collections using the <jats:styled-content style=\"fixed-case\">CMNC<\/jats:styled-content> device only.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparable collection efficiencies as well as target cell yields could be achieved with the Spectra Optia <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>\u2010 versus <jats:styled-content style=\"fixed-case\">CMNC<\/jats:styled-content> program. Similar numbers of <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>, T, B and <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content> cells could be collected with both devices. This led to a more than twofold lymphocyte recruitment from lymphatic tissue into the blood during apheresis. However, significantly more blood had to be processed with longer procedure time using the <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content> program resulting in larger product volumes compared to the <jats:styled-content style=\"fixed-case\">CMNC<\/jats:styled-content> setting. Red blood cell and platelet (<jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>) contamination were similar. Lowering the centrifugation force from <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>4\u00b75 to <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>4\u00b70 significantly reduced <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> contamination without affecting target cell yield in the product.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The Spectra Optia<jats:sup>\u00ae<\/jats:sup> <jats:styled-content style=\"fixed-case\">CMNC<\/jats:styled-content> device using lower centrifugal force (<jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>4\u00b70) showed similar target cell yield and composition as well as collection efficiencies with superior performance parameters and lower <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> contamination compared to the <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content> setting.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12540","type":"journal-article","created":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T03:16:45Z","timestamp":1502767005000},"page":"586-594","source":"Crossref","is-referenced-by-count":32,"title":["Evolution of <scp>MNC<\/scp> and lymphocyte collection settings employing different Spectra Optia<sup>\u00ae<\/sup> Leukapheresis systems"],"prefix":"10.1111","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4803-2402","authenticated-orcid":false,"given":"M.","family":"Punzel","sequence":"first","affiliation":[{"name":"MediaPark Klinik Koeln Cellex Collection Center Koeln Germany"},{"name":"Praxis Dr. Punzel Straelen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kozlova","sequence":"additional","affiliation":[{"name":"MediaPark Klinik Koeln Cellex Collection Center Koeln Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Quade","sequence":"additional","affiliation":[{"name":"Labor MVZ Quade Koeln Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. H.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"DKMS German Bone Marrow Donor Center T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Terumo BCT Lakewood CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12506"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03787.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12102"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2013.02.022"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12065"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.1021"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21319"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21463"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12799"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04202.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.03406.x"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvox.12540","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.12540","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T13:50:41Z","timestamp":1696168241000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.12540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":11,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/vox.12540"],"URL":"https:\/\/doi.org\/10.1111\/vox.12540","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]}}}