{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:33:43Z","timestamp":1791048823787,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T00:00:00Z","timestamp":1503014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science and Technology Project of Guangzhou City"},{"name":"Guangzhou Science Technology and Innovation Commission","award":["201607010027"],"award-info":[{"award-number":["201607010027"]}]},{"name":"Key Medical Disciplines and Specialities Program of Guangzhou"},{"name":"Health and Family Planning Commission of Guangzhou Municipality in China"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Several comprehensive genotyping platforms for determining red blood cell (RBC) antigens have been established and validated for use in the Caucasian and Black populations, but not for the Chinese. The multiplex ligation\u2010dependent probe amplification (MLPA) assay was validated for <jats:italic>RHD<\/jats:italic> genotyping in the Chinese.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The blood samples of 200 D+, 200 D\u2212 and 62 D variant Chinese donors were collected. RhD antigen was routinely typed by serological method. D variant phenotype was determined by an anti\u2010D panel (D\u2010Screen), when RBCs were available. The <jats:italic>RHD<\/jats:italic> genotype and its zygosity were analysed with the <jats:italic>RH<\/jats:italic>\u2010MLPA technique. When the MLPA was unable to identify a <jats:italic>RHD<\/jats:italic> variant, direct sequencing of all exons of the <jats:italic>RHD<\/jats:italic> gene was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 200 D+ donors, <jats:italic>DD<\/jats:italic> (168\/200, 84%), <jats:italic>D<\/jats:italic> (12\/200, 6%), <jats:italic>DDD<\/jats:italic> genotype (1\/200) and <jats:italic>D<\/jats:italic> variant allele carriers (19\/200, 9\u00b75%) were found. In 200 D\u2212 donors, six reported <jats:italic>RHD<\/jats:italic> alleles, <jats:italic>RHD*01EL.01<\/jats:italic>,<jats:italic> RHD*01N.03<\/jats:italic>,<jats:italic> RHD*01N.05<\/jats:italic>,<jats:italic> RHD*01N.16<\/jats:italic>,<jats:italic> RHD*DFR2<\/jats:italic> and <jats:italic>RHD*weak partial 15<\/jats:italic> and one novel <jats:italic>RHD*1154T<\/jats:italic> allele were identified in 36\u00b75% (73\/200) of them. In 62 D variant donors, three novel <jats:italic>RHD<\/jats:italic> alleles, <jats:italic>RHD*79_81delCTC<\/jats:italic>,<jats:italic> RHD*710T<\/jats:italic> and <jats:italic>RHD*689A<\/jats:italic>, and twelve reported alleles, <jats:italic>RHD*DVI.3<\/jats:italic>,<jats:italic> RHD*weak partial 15<\/jats:italic>,<jats:italic> RHD*DVI.4<\/jats:italic>,<jats:italic> RHD*01EL.01, RHD*01N.03, RHD*DLO<\/jats:italic>,<jats:italic> RHD*DV.5<\/jats:italic>,<jats:italic> RHD*D\u2010CE(2\u201010)<\/jats:italic>,<jats:italic> RHD*730C<\/jats:italic>,<jats:italic> RHD*weak D type 25<\/jats:italic>,<jats:italic> 33<\/jats:italic> and <jats:italic>72<\/jats:italic>, were identified, either alone or in combination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:italic>RH<\/jats:italic>\u2010MLPA assay correctly identified the common <jats:italic>RHD<\/jats:italic> variant alleles in the Chinese population. However, DNA sequencing was required to identify certain alleles; probes to detect these alleles should be added into the assay.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12554","type":"journal-article","created":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T10:39:43Z","timestamp":1503052783000},"page":"660-670","source":"Crossref","is-referenced-by-count":31,"title":["<i>RHD<\/i> genotype and zygosity analysis in the Chinese Southern Han D+, D\u2212 and D variant donors using the multiplex ligation\u2010dependent probe amplification assay"],"prefix":"10.1111","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6792-5612","authenticated-orcid":false,"given":"Y. L.","family":"Ji","sequence":"first","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"},{"name":"Sanquin Research and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Z.","family":"Wen","sequence":"additional","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Haer\u2010Wigman","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Veldhuisen","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"},{"name":"The Department of Immunohematology Diagnostics Sanquin Diagnostic Services Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Ligthart","sequence":"additional","affiliation":[{"name":"The Department of Immunohematology Diagnostics Sanquin Diagnostic Services Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lod\u00e9n\u2010van Straaten","sequence":"additional","affiliation":[{"name":"MRC\u2010Holland b.v. Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. S.","family":"Fu","sequence":"additional","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. E.","family":"van der Schoot","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. P.","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangzhou Blood Center Institute of Clinical Blood Transfusion Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04319.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01309.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01178.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02377.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12715"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(15)00090-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03919.x"},{"key":"e_1_2_7_9_1","first-page":"396","article-title":"A comprehensive investigation of RHD polymorphisms in the Chinese Han population in Xi'an","volume":"12","author":"Ye SH","year":"2014","journal-title":"Blood Transfus"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02181.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2005.00584.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2002.00192.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2006.00886.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12272"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.12.3662.012k12_3662_3668"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13709"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.i5789"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2009.01181.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03544.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12828"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.1.385"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13083"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03266.x"},{"key":"e_1_2_7_24_1","first-page":"3251","article-title":"Molecular basis of weak D and DEL in Han population in Anhui Province, China","volume":"125","author":"Chen Q","year":"2012","journal-title":"Chin Med J (Engl)"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12797"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.01138.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-003-0711-4"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12275"},{"key":"e_1_2_7_29_1","first-page":"103","article-title":"An identification of a new weak D phenotype","volume":"14","author":"Wu XY","year":"2012","journal-title":"J Clin Transfus Lab Med"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-125"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.04063.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000370217"},{"key":"e_1_2_7_33_1","first-page":"31","article-title":"Two novel RhD variants caused by triplet deletions in the RHD gene","volume":"17","author":"Tilley L","year":"2007","journal-title":"Transfus Med"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02221.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02856.x"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvox.12554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.12554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T17:45:58Z","timestamp":1696009558000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.12554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,18]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/vox.12554"],"URL":"https:\/\/doi.org\/10.1111\/vox.12554","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,18]]}}}