{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:18:29Z","timestamp":1774462709680,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T00:00:00Z","timestamp":1508112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>A lateral flow assay for simultaneous blood group typing of <jats:styled-content style=\"fixed-case\">ABO<\/jats:styled-content>, RhD, C, E, c, e, Cw and K with stable end\u2010point and without centrifugation is in routine use since several years (<jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>multicard<jats:sup>\u00ae<\/jats:sup>).<\/jats:p><jats:p>The typing of extended phenotype parameters belonging to the Duffy, Kidd, <jats:styled-content style=\"fixed-case\">MNS<\/jats:styled-content>s blood group systems and others, however, has not yet been demonstrated for this technique.<\/jats:p><jats:p>Reliable detection of Fy<jats:sup>x<\/jats:sup>, a weak Fy<jats:sup>b<\/jats:sup> phenotype with a pronounced quantitative reduction of the number of Fy<jats:sup>b<\/jats:sup> antigens on the erythrocyte surface, remains a weakness of current serological blood grouping techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The performance characteristics of the following reagents were evaluated in donor and patient samples in lateral flow technology (<jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>multicard<jats:sup>\u00ae<\/jats:sup>): Anti\u2010Fy<jats:sup>a<\/jats:sup>, \u2010Fy<jats:sup>b<\/jats:sup>, \u2010Jk<jats:sup>a<\/jats:sup>, \u2010Jk<jats:sup>b<\/jats:sup>, \u2010S, \u2010s\u0305, \u2010P1 and \u2010k. The sensitivity to detect Fy<jats:sup>x<\/jats:sup> was in addition evaluated with Fy<jats:sup>x<\/jats:sup> positive samples, which had been preselected by <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content>\u2010based genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All results obtained with the <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>multicard<jats:sup>\u00ae<\/jats:sup> were in full accordance with those of the <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010certified reference products for all the eight reagent formulations used: Anti\u2010Fy<jats:sup>a<\/jats:sup>, \u2010Fy<jats:sup>b<\/jats:sup>, \u2010Jk<jats:sup>a<\/jats:sup>, \u2010Jk<jats:sup>b<\/jats:sup>, \u2010S, \u2010s\u0305, \u2010P1 and \u2013k. Also, all Fy<jats:sup>x<\/jats:sup> phenotypes of the selected population of 93 positive samples, originally identified by <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content>\u2010based genotyping, were reliably detected by the lateral flow assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Extended phenotype blood group parameters, including the serologically challenging Fy<jats:sup>x<\/jats:sup> phenotype, can be determined simultaneously, rapidly and accurately using the lateral flow (<jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>multicard<jats:sup>\u00ae<\/jats:sup>) technology, even in cases when IgG class antibodies are the only source of diagnostic antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12603","type":"journal-article","created":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T04:42:40Z","timestamp":1508215360000},"page":"177-184","source":"Crossref","is-referenced-by-count":11,"title":["A uniform method for the simultaneous blood group phenotyping of Fy<sup>a<\/sup>, Fy<sup>b<\/sup>, Jk<sup>a<\/sup>, Jk<sup>b<\/sup>, S, s\u0305, P1, k applying lateral\u2010flow technique"],"prefix":"10.1111","volume":"113","author":[{"given":"A.","family":"Caesar","sequence":"first","affiliation":[{"name":"Medion Grifols Diagnostics AG Duedingen Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service Zurich Swiss Red Cross Schlieren Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Trost","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service Zurich Swiss Red Cross Schlieren Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Neuenschwander","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service Zurich Swiss Red Cross Schlieren Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Geisen","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine and Immunohaematology German Red Cross Blood Donor Service Baden\u2010W\u00fcrttemberg\u2010Hessen gGmbH Goethe University Hospital Frankfurt am Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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