{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T10:43:19Z","timestamp":1773744199979,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T00:00:00Z","timestamp":1570320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>Weak D phenotypes with very low antigen densities and DEL phenotype may not be detected in RhD typing routine and could be typed as D\u2010negative, leading to D alloimmunization of D\u2010negative recipients. The present study aimed to investigate the presence of <jats:italic>RHD<\/jats:italic>\u2010positive genotypes in blood donors typed as D\u2010negative by an automated system using the solid\u2010phase methodology as a confirmatory test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two screenings were performed in different selected donor populations. For the first screening, we selected 1403 blood donor samples typed as D\u2010negative regardless of the CE status, and in the second screening, we selected 517 donor samples typed as D\u2010negative C+ and\/or E+. RhD typing was performed by microplate in an automated equipment (Neo\u2010Immucor\u00ae), and the confirmatory test was performed by solid\u2010phase technique using Capture R\u00ae technology. A multiplex PCR specific to <jats:italic>RHD<\/jats:italic> and <jats:italic>RHD\u03c8<\/jats:italic> was performed in a pool of 6 DNA samples. Sequencing of <jats:italic>RHD<\/jats:italic> exons was performed in all <jats:italic>RHD<\/jats:italic>\u2010positive samples, and a specific PCR was used to identify the <jats:italic>D\u2010CE(4\u20107)\u2010D<\/jats:italic> hybrid gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and conclusion<\/jats:title><jats:p>No weak D type was found in either screening populations. Additionally, 353 (18\u00b74%) D\u2010negative samples presented previously reported non\u2010functional <jats:italic>RHD<\/jats:italic> genes, 2 samples had a DEL allele, and 6 samples demonstrated new alleles, including one novel DEL allele. Our study identified six new <jats:italic>RHD<\/jats:italic> alleles and showed that the inclusion of a confirmatory test using serological methodology with high sensitivity can reduce the frequency of weak D samples typed as D\u2010negative.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12851","type":"journal-article","created":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T07:59:27Z","timestamp":1570348767000},"page":"869-875","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of <i>RHD<\/i> alleles present in serologically <i>RHD<\/i>\u2010negative donors determined by a sensitive microplate technique"],"prefix":"10.1111","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2812-4238","authenticated-orcid":false,"given":"Tatiane Aparecida","family":"de Paula Vendrame","sequence":"first","affiliation":[{"name":"Colsan\u2010 Associa\u00e7\u00e3o Beneficente de Coleta de Sangue S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5270-3953","authenticated-orcid":false,"given":"Carine","family":"Prisco Arnoni","sequence":"additional","affiliation":[{"name":"Colsan\u2010 Associa\u00e7\u00e3o Beneficente de Coleta de Sangue S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana\u00edna","family":"Guilhem Muniz","sequence":"additional","affiliation":[{"name":"Hemocentro\u2010 S\u00e3o Jos\u00e9 do Rio Preto S\u00e3o Jos\u00e9 do Rio Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosangela","family":"de Medeiros Person","sequence":"additional","affiliation":[{"name":"Colsan\u2010 Associa\u00e7\u00e3o Beneficente de Coleta de Sangue S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afonso Jos\u00e9","family":"Pereira Cortez","sequence":"additional","affiliation":[{"name":"Colsan\u2010 Associa\u00e7\u00e3o Beneficente de Coleta de Sangue S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia","family":"Roche Moreira Latini","sequence":"additional","affiliation":[{"name":"Colsan\u2010 Associa\u00e7\u00e3o Beneficente de Coleta de Sangue S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian","family":"Castilho","sequence":"additional","affiliation":[{"name":"Hemocentro \u2010 Unicamp Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,6]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"50","article-title":"The genetics of the Rhesus blood group system","volume":"5","author":"Flegel WA","year":"2007","journal-title":"Blood Transfus"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2006.09.010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004390051024"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3206"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V78.10.2747.2747"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.1.12"},{"key":"e_1_2_7_8_1","first-page":"396","article-title":"A comprehensive investigation of RHD polymorphisms in the Chinese Han population in Xi'an","volume":"12","author":"Ye SH","year":"2014","journal-title":"Blood Transfus"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-2-10"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01975.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1984.24685066827.x"},{"key":"e_1_2_7_12_1","unstructured":"WagnerFF FlegelW:The human RhesusBase version 2.0.http:\/\/www.rhesusbase.info\/ 2018. 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