{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:08:36Z","timestamp":1747188516296,"version":"3.40.5"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,10]],"date-time":"2019-11-10T00:00:00Z","timestamp":1573344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BA-5158-1"],"award-info":[{"award-number":["BA-5158-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012940","name":"Universit\u00e4tsklinikum T\u00fcbingen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012940","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>Platelet transfusion is a standard medical therapy used to treat several bleeding disorders. However, a critical drawback is the dependency on donor\u2010derived platelets, which leads to concerns like insufficient availability and immunological complications. In vitro platelet production from hematopoietic progenitor cells (CD34) may represent a reasonable solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>CD34+ cells were isolated from either buffy coat or peripheral blood and compared in terms of platelet production in vitro. The number and the quality of magnetically isolated CD34+ cells and their capability to differentiate into mature megakaryocytes were investigated using flow cytometry. Additionally, the functionality of megakaryocytes in term of in vitro platelet production was tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Similar purity and quantity of CD34+ cells was found after their isolation from both cell sources. In contrast, after 6\u00a0days of culture, enhanced number of CD34+ cells isolated from buffy coat compared with peripheral blood was observed (5\u00b73\u00a0x\u00a0106 vs. 3\u00b70\u00a0x\u00a0106, respectively). Interestingly, despite a comparable nuclear maturation phenotype, the yield of platelets released from buffy coat\u2010derived megakaryocytes was significantly higher than from peripheral blood cells (platelet yield pro MK: 7\u00b72 vs. 2\u00b77, respectively). Importantly, platelets produced from buffy coat\u2010derived cells could be activated by agonists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Haematopoietic progenitor cells isolated from buffy coat have increased yield of platelets released from mature megakaryocytes and enhanced in vitro functionality, compared with peripheral blood\u2010derived cells. Our study, suggests that buffy coat, obtained during blood donation processing, might be a promising source of megakaryocytes for in vitro platelet production.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12863","type":"journal-article","created":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T06:04:55Z","timestamp":1573452295000},"page":"94-102","source":"Crossref","is-referenced-by-count":4,"title":["Blood donor\u2010derived buffy coat to produce platelets in vitro"],"prefix":"10.1111","volume":"115","author":[{"given":"Irene","family":"Marini","sequence":"first","affiliation":[{"name":"Medical Faculty of T\u00fcbingen University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavianna","family":"Rigoni","sequence":"additional","affiliation":[{"name":"Medical Faculty of T\u00fcbingen University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Zlamal","sequence":"additional","affiliation":[{"name":"Medical Faculty of T\u00fcbingen University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisann","family":"Pelzl","sequence":"additional","affiliation":[{"name":"Medical Faculty of T\u00fcbingen University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"Althaus","sequence":"additional","affiliation":[{"name":"Center for Clinical Transfusion Medicine T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Nowak\u2010Harnau","sequence":"additional","affiliation":[{"name":"Center for Clinical Transfusion Medicine T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew T.","family":"Rondina","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program University of Utah Salt Lake City UT USA"},{"name":"Departments of Internal Medicine and Pathology University of Utah Salt Lake City UT USA"},{"name":"Department of Medicine and GRECC George E. Wahlen VAMC Salt Lake City UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6797-6812","authenticated-orcid":false,"given":"Tamam","family":"Bakchoul","sequence":"additional","affiliation":[{"name":"Medical Faculty of T\u00fcbingen University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"Center for Clinical Transfusion Medicine T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-575340"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2016.1148806"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61198-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.2.402.402"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-144956"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2010.03.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201410052"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06215.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-088500"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm973"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2384-1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2014.02.002"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12764"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0111"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13736"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-09-670208"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.161562"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1646493"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13547"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11208"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2014-0183"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2080"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74556"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1191536"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0125057"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43326"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-209205"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-01-547927"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/09537104.2016.1257783"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13375"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.25477"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-574913"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.12863","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.12863","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.12863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T18:29:46Z","timestamp":1693852186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.12863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,10]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/vox.12863"],"URL":"https:\/\/doi.org\/10.1111\/vox.12863","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"type":"print","value":"0042-9007"},{"type":"electronic","value":"1423-0410"}],"subject":[],"published":{"date-parts":[[2019,11,10]]}}}