{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:42:24Z","timestamp":1787920944255,"version":"build-2784847793"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T00:00:00Z","timestamp":1582156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009425","name":"Landsteiner Foundation for Blood Transfusion Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009425","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004350","name":"Studienstiftung des Deutschen Volkes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004350","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In adult immune thrombocytopenia (ITP), an acquired autoimmune bleeding disorder, anti\u2010platelet autoantibody testing may be useful as a rule\u2010in test. Childhood ITP has different disease characteristics, and the diagnostic and prognostic value of anti\u2010platelet antibody testing remains uncertain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To systematically review the diagnostic accuracy of anti\u2010platelet autoantibody testing in childhood ITP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>PubMed and EMBASE were searched for studies evaluating immunoassays in childhood ITP. Study quality was assessed (QUADAS2), and evidence was synthesized descriptively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In total, 40 studies (1606 patients) were identified. Nine studies reported sufficient data to determine diagnostic accuracy measures. Anti\u2010platelet IgG antibody testing showed a moderate sensitivity (0\u00b736\u20130\u00b780 platelet\u2010associated IgG [direct test]; 0\u00b719\u20130\u00b739 circulating IgG [indirect test]). In studies that reported control data, including patients with non\u2010immune thrombocytopenia, specificity was very good (0\u00b780\u20131\u00b700). Glycoprotein\u2010specific immunoassays showed comparable sensitivity (three studies) and predominantly identified IgG anti\u2010GP IIb\/IIIa antibodies, with few IgG anti\u2010GP Ib\/IX antibodies. Anti\u2010platelet IgM antibodies were identified in a substantial proportion of children (sensitivity 0\u00b762\u20130\u00b764 for direct and indirect tests).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The diagnostic evaluation of IgG and IgM anti\u2010platelet antibodies may be useful as a rule\u2010in test for ITP. In children with insufficient platelets for a direct test, indirect tests may be performed instead. A negative test does not rule out the diagnosis of ITP. Future studies should evaluate the value of anti\u2010platelet antibody tests in thrombocytopenic children with suspected ITP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.12894","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T20:19:08Z","timestamp":1582229948000},"page":"323-333","source":"Crossref","is-referenced-by-count":19,"title":["Anti\u2010platelet antibody immunoassays in childhood immune thrombocytopenia: a systematic review"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5555-376X","authenticated-orcid":false,"given":"David E.","family":"Schmidt","sequence":"first","affiliation":[{"name":"Sanquin Research Department of Experimental Immunohematology Amsterdam The Netherlands"},{"name":"Landsteiner Laboratory Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke J.","family":"Lakerveld","sequence":"additional","affiliation":[{"name":"Sanquin Research Department of Experimental Immunohematology Amsterdam The Netherlands"},{"name":"Landsteiner Laboratory Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja M. 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