{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:25:05Z","timestamp":1770823505791,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T00:00:00Z","timestamp":1583971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>Fetal <jats:italic>RHD<\/jats:italic> genotyping of cell\u2010free maternal plasma DNA from RhD negative pregnant women can be used to guide targeted antenatal and postnatal anti\u2010D prophylaxis for the prevention of RhD immunization. To assure the quality of clinical testing, we conducted an external quality assessment workshop with the participation of 31 laboratories.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Aliquots of pooled maternal plasma from gestational week 25 were sent to each laboratory. One sample was fetal <jats:italic>RHD<\/jats:italic> positive, and a second sample was fetal <jats:italic>RHD<\/jats:italic> negative. A reporting scheme was supplied for data collection, including questions regarding the methodological setup, results and clinical recommendations. The samples were tested blindly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Different methodological approaches were used; 29 laboratories used qPCR and two laboratories used ddPCR, employing a total of eight different combinations of <jats:italic>RHD<\/jats:italic> exon targets. Fetal <jats:italic>RHD<\/jats:italic> genotyping was performed with no false\u2010negative and no false\u2010positive results. One inconclusive result was reported for the <jats:italic>RHD<\/jats:italic> positive sample. All clinical conclusions were satisfactory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This external quality assessment workshop demonstrates that despite the different approaches taken to perform the clinical assays, fetal <jats:italic>RHD<\/jats:italic> genotyping is a reliable laboratory assay to guide targeted use of Rh prophylaxis in a clinical setting.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12908","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T02:33:25Z","timestamp":1584066805000},"page":"466-471","source":"Crossref","is-referenced-by-count":8,"title":["External quality assessment of noninvasive fetal <i>RHD<\/i> genotyping"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2487-5373","authenticated-orcid":false,"given":"Frederik Banch","family":"Clausen","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology, Rigshospitalet Copenhagen University 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