{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:41:12Z","timestamp":1770824472895,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T00:00:00Z","timestamp":1584921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003173","name":"Crafoordska Stiftelsen","doi-asserted-by":"publisher","award":["20151060"],"award-info":[{"award-number":["20151060"]}],"id":[{"id":"10.13039\/501100003173","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004063","name":"Knut och Alice Wallenbergs Stiftelse","doi-asserted-by":"publisher","award":["2014.0312"],"award-info":[{"award-number":["2014.0312"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2014\u201071X\u201014251"],"award-info":[{"award-number":["2014\u201071X\u201014251"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>The Mi(a+) GP(B\u2010A\u2010B) hybrid phenotypes occur with a prevalence of 2%\u201323% across Southeast Asia. While the s antigen is alleged to be altered, no evidence for specific variants is known. Screening using a monoclonal IgM anti\u2010s mistyped six S\u2012s+ RBC units as S\u2012s\u2012. Further, alloanti\u2010s was identified in an S+s+ patient. Our objective was to investigate the s antigen further.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>DNA from 63 Thai blood donor samples PCR\u2010positive for a <jats:italic>GYP(B\u2010A\u2010B)<\/jats:italic> hybrid was sequenced with primers spanning <jats:italic>GYPB<\/jats:italic> exons 3\u20134. Flow cytometry was used for semiquantitative analysis of s expression and correlated with the glycophorin genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DNA sequencing showed that <jats:italic>GYP*Mur<\/jats:italic> was carried by 56\/63 samples (88\u00b79%) of which 5\/56 lacked normal <jats:italic>GYPB<\/jats:italic>: three of these were <jats:italic>GYP*Mur<\/jats:italic> homozygotes, one was a compound heterozygote carrying <jats:italic>GYP*Mur<\/jats:italic> and a <jats:italic>GYP*Bun<\/jats:italic>\u2010like allele (designated <jats:italic>GYP*Thai<\/jats:italic>), and the fifth sample carried <jats:italic>GYP*Mur<\/jats:italic> and another <jats:italic>GYP*Bun<\/jats:italic>\u2010like allele. Seven samples (7\/63) were <jats:italic>GYP*Thai<\/jats:italic> heterozygotes. IgM monoclonal anti\u2010s (P3BER) did not react with the s antigen carried by GP.Mur or GP.Bun, whereas two IgG anti\u2010s showed enhanced reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We confirmed that <jats:italic>GYP*Mur<\/jats:italic> is the most frequent variant in Thai blood donors and also identified <jats:italic>GYP*Thai<\/jats:italic> with a frequency of 1\u00b71%. We showed that s antigen on Mi(a+) GP(B\u2010A\u2010B) hybrids is qualitatively altered and should be considered when selecting reagents for phenotyping where such hybrids are prevalent, endemically and in blood centres worldwide.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12909","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T08:40:24Z","timestamp":1584952824000},"page":"472-477","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of <i>GYP*Mur<\/i> and novel <i>GYP*Bun<\/i>\u2010like hybrids in Thai blood donors reveals a qualitatively altered s antigen"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6407-7109","authenticated-orcid":false,"given":"Philaiphon","family":"Jongruamklang","sequence":"first","affiliation":[{"name":"Division of Hematology and Transfusion Medicine Department of Laboratory Medicine Lund University Lund Sweden"},{"name":"Department of Medical Technology School of Allied Health Sciences University of Phayao Phayao Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shane","family":"Grimsley","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory NHSBT Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Thornton","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory NHSBT Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janine","family":"Robb","sequence":"additional","affiliation":[{"name":"Quotient Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1647-9610","authenticated-orcid":false,"given":"Martin L.","family":"Olsson","sequence":"additional","affiliation":[{"name":"Division of Hematology and Transfusion Medicine Department of Laboratory Medicine Lund University Lund Sweden"},{"name":"Clinical Immunology and Transfusion Medicine Office for Medical Services Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2940-2604","authenticated-orcid":false,"given":"Jill R.","family":"Storry","sequence":"additional","affiliation":[{"name":"Division of Hematology and Transfusion Medicine Department of Laboratory Medicine Lund University Lund Sweden"},{"name":"Clinical Immunology and Transfusion Medicine Office for Medical Services Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118493595"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14624"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.1967.tb05516.x"},{"key":"e_1_2_8_5_1","first-page":"101","article-title":"Detection of MNS hybrid molecules (GP.Hut, GP.Mur, GP.Hop, GP.Bun 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