{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:31:28Z","timestamp":1767857488226,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>Background and objective<\/jats:title><jats:p>In the WHO Universal test and treat strategy, false\u2010positive HIV blood donors and patients may be unnecessarily put under antiretroviral treatment and false\u2010negative subjects may be lost to follow\u2010up. This study assessed the false positivity rate of the Cameroonian national HIV screening testing algorithm and the benefit of a confirmation test in the enrolment of patients and donors in the HIV care programme.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included initial HIV reactive blood donors and patients in a cross\u2010sectional study conducted in two Cameroonian hospitals. Samples were retested according to the Cameroon national algorithm for HIV diagnosis. A positive or discordant sample was retested with the Geenius Bio\u2010Rad HIV 1&amp;2 (Bio\u2010Rad, Marnes\u2010la\u2010Coquette, France) for confirmation. The Geenius HIV\u20101\u2010positive results with \u2018poor\u2019 profiles were retested for RNA as well as the Geenius indeterminate results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 356 participants, 190\/225 (84\u00b74%) patients and 76\/131 (58%) blood donors were declared positive with the national algorithm; 257 participants (96\u00b76%) were confirmed HIV\u20101\u2010positive. The study revealed that about 34\/1000 blood donors and patients are false\u2010positive and unnecessarily put on treatment; 89\/1000 blood donors and patients declared discordant could have been included immediately in the HIV care programme if confirmatory testing was performed. The second test of the algorithm had a false\u2010negative rate of 3%. Eleven samples (3\u00b71%) were Geenius poor positive and NAT negative.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The universal test and treat strategy may identify and refer more individuals to HIV care if a third rapid confirmatory test is performed for discordant cases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12942","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T02:13:51Z","timestamp":1590718431000},"page":"686-694","source":"Crossref","is-referenced-by-count":5,"title":["The use of the Geenius<sup>TM<\/sup> HIV\u20101\/2 Rapid confirmatory test for the enrolment of patients and blood donors in the WHO Universal Test and Treat Strategy in Cameroon, Africa"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2179-3105","authenticated-orcid":false,"given":"Claude T.","family":"Tagny","sequence":"first","affiliation":[{"name":"Hematology and Blood Transfusion Service Yaound\u00e9 University Hospital Yaound\u00e9 Cameroon"},{"name":"Faculty of Medicine and Biomedical Sciences UY1 Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Bissim","sequence":"additional","affiliation":[{"name":"School of Health Sciences Catholic University of Central Africa Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolande","family":"Djeumen","sequence":"additional","affiliation":[{"name":"School of Health Sciences Catholic University of Central Africa Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise","family":"Ngo Sack","sequence":"additional","affiliation":[{"name":"Blood Bank of the Yaound\u00e9 Central Hospital Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prisca","family":"Angandji","sequence":"additional","affiliation":[{"name":"Yaound\u00e9 Jamot Hospital Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annick","family":"Ndoumba","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Biomedical Sciences UY1 Yaound\u00e9 Cameroon"},{"name":"School of Health Sciences Catholic University of Central Africa Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Kouanfack","sequence":"additional","affiliation":[{"name":"Yaound\u00e9 Central Hospital Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Eno","sequence":"additional","affiliation":[{"name":"The US Center for Diseases\u2019 Control Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dora","family":"Mbanya","sequence":"additional","affiliation":[{"name":"Hematology and Blood Transfusion Service Yaound\u00e9 University Hospital Yaound\u00e9 Cameroon"},{"name":"Faculty of Medicine and Biomedical Sciences UY1 Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward L.","family":"Murphy","sequence":"additional","affiliation":[{"name":"University of California San Francisco CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syria","family":"Laperche","sequence":"additional","affiliation":[{"name":"National Institute of Blood Transfusion Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,28]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"University of Columbia US.Cameroon Population\u2010based HIV impact assessment (CAMPHIA). 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