{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T13:14:33Z","timestamp":1770729273234,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>The aims of the 19th International Society of Blood Transfusion Platelet Immunology Workshop were to compare the sensitivity and specificity of in\u2010house and commercially available methods for the detection of alloantibodies against human platelet antigens. Survey regarding laboratory management of samples collected for the diagnosis of foetal neonatal alloimmune thrombocytopenia was also conducted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Twenty\u2010nine laboratories from 17 countries were invited to participate. Seven serum or plasma samples for antibody identification and eight DNA samples for genotyping were sent to participating laboratories. Additionally, samples, critical reagents, materials and instructions for three exercises, one using a commercial kit (Pak\u00a0Lx), one on platelet preparation for the detection of anti\u2010HPA\u20103 antibodies and one for testing four anti\u2010CD109 monoclonal antibodies for anti\u2010HPA\u201015 antibody detection, were provided.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anti\u2010HPA\u20101a, anti\u2010HPA\u20102b, anti\u2010HPA\u20105b and anti\u2010GPIV were detected by the majority of the 28 reporting laboratories using their respective in\u2010house MAIPA assay and\/or a commercially available assay. Conversely, very few laboratories correctly identified anti\u2010HPA\u20103a and HPA\u201015b. DNA genotyping of HPA and HLA alleles was highly accurate, with just a few discrepancies relative to the expected results. The Pak\u00a0Lx kit has proven reliable for detecting anti\u2010HPA\u20101a, anti\u2010HPA\u20105a and anti\u2010HLA; however, it failed at identifying an anti\u2010HPA\u20103a in a clinical sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Some anti\u2010platelet alloantibodies are reliably and consistently detected, yet others remain difficult to detect. Genotyping of HPA and HLA alleles has proven to be highly accurate and robust. Future work should focus on optimizing the detection of anti\u2010HPA\u20103 and anti\u2010HPA\u201015 antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.12945","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T05:31:33Z","timestamp":1590730293000},"page":"767-782","source":"Crossref","is-referenced-by-count":4,"title":["Report on the 19th International Society of Blood Transfusion Platelet Immunology Workshop 2018"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-4198","authenticated-orcid":false,"given":"Antoine","family":"Lewin","sequence":"first","affiliation":[{"name":"Medical Affairs and Innovation H\u00e9ma\u2010Qu\u00e9bec Saint\u2010Laurent QC Canada"},{"name":"Obstetrics and Gynecology University of Sherbrooke Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shadhiya","family":"Al Khan","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynnette","family":"Beaudin","sequence":"additional","affiliation":[{"name":"Diagnostic Services Canadian Blood Services Edmonton AB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynne","family":"Meilleur","sequence":"additional","affiliation":[{"name":"Diagnostic Services Canadian Blood Services Edmonton AB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-3378","authenticated-orcid":false,"given":"Gwen","family":"Clarke","sequence":"additional","affiliation":[{"name":"Diagnostic Services Canadian Blood Services Edmonton AB Canada"},{"name":"Laboratory Medicine and Pathology University of Alberta Edmonton AB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Richard","sequence":"additional","affiliation":[{"name":"Medical Affairs and Innovation H\u00e9ma\u2010Qu\u00e9bec Saint\u2010Laurent QC Canada"},{"name":"Stem Cell and Reference Laboratories H\u00e9ma\u2010Qu\u00e9bec Saint\u2010Laurent QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,28]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"IATA.IATA Dangerous Goods Regulations 58th Edition Packing Instructions 650[Internet]. 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