{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:06:50Z","timestamp":1747188410346,"version":"3.40.5"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>Blood donors might develop iron deficiency as approximately 250\u00a0mg of iron is lost with every donation. Susceptibility to iron deficiency and low haemoglobin levels differs between individuals, which might be due to genetic variation. Therefore, the aim of this study was to investigate associations between single nucleotide polymorphisms (SNPs) and haemoglobin trajectories, haemoglobin levels and ferritin levels in blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>In 2655 donors participating in the observational cohort study Donor InSight\u2010III (2015\u20132017), haemoglobin and ferritin levels were measured in venous EDTA whole blood and plasma samples, respectively. Haemoglobin trajectories (stable\/declining) were determined by fitting growth\u2010mixture models on repeated pre\u2010donation capillary haemoglobin measurements. Genotyping was done using the UK Biobank \u2013 version 2 Axiom Array. Single SNP analyses adopting an additive genetic model on imputed genetic variants were performed for haemoglobin trajectories, haemoglobin levels and ferritin levels. Conditional analyses identified independent SNPs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twelve, twenty and twenty\u2010four independent SNPs were associated with haemoglobin trajectories, haemoglobin levels and ferritin levels respectively (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a01\u00a0x\u00a010<jats:sup>\u22125<\/jats:sup>). Rs112016443 reached genome\u2010wide significance for ferritin levels, which influences <jats:italic>WDSUB1<\/jats:italic> expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Rs112016443 was genome\u2010wide significantly associated with ferritin levels in Dutch donors. Further validation studies are needed, as well as studies towards underlying mechanisms and predicting iron deficiency using SNPs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13066","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T18:39:51Z","timestamp":1611599991000},"page":"755-765","source":"Crossref","is-referenced-by-count":6,"title":["Genetic determinants of ferritin, haemoglobin levels and haemoglobin trajectories: results from Donor InSight"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2253-9683","authenticated-orcid":false,"given":"Tiffany","family":"Timmer","sequence":"first","affiliation":[{"name":"Department of Donor Medicine Research \u2010 Donor Studies Sanquin Research Amsterdam The Netherlands"},{"name":"Department of Public Health Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"},{"name":"Landsteiner Laboratory Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Tanck","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology, Biostatistics and Bioinformatics Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Penkett","sequence":"additional","affiliation":[{"name":"Department of Haematology Cambridge Biomedical Campus University of Cambridge Cambridge UK"},{"name":"NIHR BioResource Cambridge Biomedical Campus Cambridge University Hospitals Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Stirrups","sequence":"additional","affiliation":[{"name":"Department of Haematology Cambridge Biomedical Campus University of Cambridge Cambridge UK"},{"name":"NIHR BioResource Cambridge Biomedical Campus Cambridge University Hospitals Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Gleadall","sequence":"additional","affiliation":[{"name":"Department of Haematology Cambridge Biomedical Campus University of Cambridge Cambridge UK"},{"name":"Cambridge Biomedical Campus NHS Blood and Transplant Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wim","family":"de Kort","sequence":"additional","affiliation":[{"name":"Department of Donor Medicine Research \u2010 Donor Studies Sanquin Research Amsterdam The Netherlands"},{"name":"Department of Public Health Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"van der Schoot","sequence":"additional","affiliation":[{"name":"Landsteiner Laboratory Amsterdam UMC University of Amsterdam Amsterdam The Netherlands"},{"name":"Department of Experimental Immunohematology Sanquin Research Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"van den Hurk","sequence":"additional","affiliation":[{"name":"Department of Donor Medicine Research \u2010 Donor Studies Sanquin Research Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12327"},{"volume-title":"Minerals and trace elements. 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