{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:09:11Z","timestamp":1771459751021,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T00:00:00Z","timestamp":1624406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Large\u2010scale single nucleotide variation (SNV)\u2010based blood group genotyping assays have been made available for over a decade. Due to differences in ethnic groups, there is much diversity in clinically important blood group antigens and genetic variants. Here, we developed a robust matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight (MALDI\u2010TOF)\u2010based blood group genotyping method on MassARRAY system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A total of 1428 donors were enrolled into three groups: (a) reagent red cell donors; (b) rare donor or common antigen\u2010negative donors; and (c) group O, R<jats:sub>1<\/jats:sub>R<jats:sub>1<\/jats:sub>\/R<jats:sub>2<\/jats:sub>R<jats:sub>2<\/jats:sub> donors. Forty\u2010two SNVs were designed for determining nine blood groups, with X\/Y chromosome in two multiplex reactions, on MassARRAY 96\u2010well format system. Further targeted sequence analyses were performed by Sanger sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>WHO reference reagent (NIBSC code: 11\/214) was tested for concordance with the provided genotype results. Among the donors, concordance rate was over 99%. Alleles of important phenotypes such as Mi(a+), Di(a+), and Asian\u2010type DEL and alleles of rare blood groups such as Fy(a\u2212), Jk(a\u2212b\u2212) and s\u2212 were screened. Three types of discrepancies were found. Serologically, the \u2018N\u2019 antigen was expressed on genetically <jats:italic>MM<\/jats:italic> with <jats:italic>GYP*Mur<\/jats:italic> red blood cells and caused genuine discrepancies (9.5%). Genetically, allele dropout (ADO) was caused by rare SNV in the primer for <jats:italic>Ss<\/jats:italic> genotype (2.1%) and partial insertion of <jats:italic>RHD<\/jats:italic> genes (0.9%) led to difficulties in predicting phenotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hemo panel module and MassARRAY System in 96\u2010well format showed good performance in terms of large\u2010scale blood group genotyping and phenotype predictions. Implementation of this method is effective for routine blood group genotype screening of donors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13133","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T07:25:07Z","timestamp":1624433107000},"page":"99-108","source":"Crossref","is-referenced-by-count":3,"title":["The efficacy of ethnic specific blood groups genotyping for routine donor investigation and rare donor identification in Taiwan"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9280-0803","authenticated-orcid":false,"given":"Meng\u2010Hua","family":"Yang","sequence":"first","affiliation":[{"name":"Head Office Taiwan Blood Services Foundation  Taipei Taiwan"},{"name":"Institute of Clinical Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jen\u2010Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Head Office 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