{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:21:21Z","timestamp":1790774481147,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Balancing blood supply safety and sufficiency is challenging in malaria\u2010endemic countries where the risk of transfusion\u2010transmitted malaria (TTM) is ever\u2010present. In support of reducing this risk, our study aimed at evaluating the performance of the Sysmex XN\u201031 analyser in blood donor malaria screening, as compared with current practice in Malawi.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>This prospective observational study was conducted on remnant venous donor blood samples collected at Malawi Blood Transfusion Service donation sites countrywide for routine blood\u2010borne pathogen screening. XN\u201031 results were compared with routine thick smear malaria microscopy, using expert microscopy (phase 1 and 2) plus qualitative malaria polymerase chain reaction (PCR) (phase 2) to adjudicate discrepancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>XN\u201031 detected malaria in 614 (11.6%) of 5281 study samples compared with 341 (6.5%) for routine microscopy. Of the 273 discrepant samples, 60 smears (phase 1) could not be retrieved for expert microscopic review. Expert microscopy confirmed the XN\u201031 positivity in 78.8% (149\/189) and 91.7% (22\/24) of discrepant samples in phase 1 (<jats:italic>n<\/jats:italic>\u00a0=\u20094416) and phase 2 (<jats:italic>n<\/jats:italic>\u00a0=\u2009975), respectively, with two cases requiring PCR testing, confirming one each as positive and negative, giving sensitivities of 100% and 75% and specificities of 99.9% and 100%, respectively, for XN\u201031 and routine microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The automated Sysmex XN\u201031 analyser's high sensitivity and specificity, ability to detect all <jats:italic>Plasmodium<\/jats:italic> species and high throughput with rapid turnaround\u2010time, overcomes many of the limitations of currently available diagnostic tests, making it well\u2010suited for malaria screening of donated blood in malaria\u2010endemic countries in support of TTM risk reduction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13208","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T21:53:20Z","timestamp":1632520400000},"page":"346-353","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of the Sysmex <scp>XN<\/scp>\u201031 automated analyser for blood donor malaria screening at Malawi Blood Transfusion Services"],"prefix":"10.1111","volume":"117","author":[{"given":"Bridon","family":"M'baya","sequence":"first","affiliation":[{"name":"Malawi Blood Transfusion Service  Blantyre Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thom","family":"Mfune","sequence":"additional","affiliation":[{"name":"Malawi Blood Transfusion Service  Blantyre Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aubrey","family":"Samon","sequence":"additional","affiliation":[{"name":"Malawi Blood Transfusion Service  Blantyre Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talent","family":"Hwandih","sequence":"additional","affiliation":[{"name":"Sysmex Europe  Norderstedt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5366-4842","authenticated-orcid":false,"given":"Marion","family":"M\u00fcnster","sequence":"additional","affiliation":[{"name":"Sysmex Europe  Norderstedt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit130"},{"key":"e_1_2_7_3_1","volume-title":"The 2016 global status report on blood safety and availability","author":"World Health Organization","year":"2017"},{"key":"e_1_2_7_4_1","first-page":"512","article-title":"Towards pathogen inactivation of red blood cells and whole blood targeting viral DNA\/RNA: design, technologies, and future prospects for developing countries","volume":"15","author":"Drew VJ","year":"2017","journal-title":"Blood Transfus"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00581-X"},{"key":"e_1_2_7_6_1","volume-title":"Screening donated blood for transfusion transmissible infections\u2013recommendations","author":"World Health Organization","year":"2010"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.30875\/60123dd4-en"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-3050-1"},{"key":"e_1_2_7_9_1","volume-title":"Post baseline situational analysis of blood safety in Malawi 2015. 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