{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:37:03Z","timestamp":1773283023629,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2023,3]]},"abstract":"Abstract<\/jats:title>Background and Objectives<\/jats:title>Data about collection efficiency 1 (CE1<\/jats:styled-content>), which takes into account blood cell counts before and after collection, thus providing a more accurate estimate, in the collection of autologous T lymphocytes by apheresis for chimeric antigen receptor (CAR<\/jats:styled-content>) T\u2010cells remain scarce. We evaluated donor\u2010 and procedure\u2010related characteristics that might influence the CE1<\/jats:styled-content> of lymphocytes.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>We retrospectively reviewed all mononuclear cell (MNC) collections) performed for CAR<\/jats:styled-content> T\u2010cell manufacturing in our institution from May 2017 to June 2021 in adult patients. Age, gender, weight, total blood volume (TBV<\/jats:styled-content>), prior haematopoietic cell transplant, diagnosis, days between last treatment and apheresis, pre\u2010collection cell counts, duration of apheresis, TBV<\/jats:styled-content> processed, vascular access, inlet flow and device type were analysed as potential factors affecting CE1<\/jats:styled-content> of lymphocytes.<\/jats:p><\/jats:sec>Results<\/jats:title>A total of 127 autologous MNC collections were performed on 118 patients diagnosed with acute lymphoblastic leukaemia (n<\/jats:italic>\u00a0=\u00a053, 45%), non\u2010Hodgkin<\/jats:styled-content> lymphoma (n<\/jats:italic>\u00a0=\u00a040, 34%), multiple myeloma (n<\/jats:italic>\u00a0=\u00a019, 16%), and chronic lymphocytic leukaemia (n<\/jats:italic>\u00a0=\u00a06, 5%). The median CE1<\/jats:styled-content> of lymphocytes was 47% (interquartile range: 32%\u201365%). In multiple regression analysis, Amicus device was associated with higher CE1<\/jats:styled-content> of lymphocytes (p<\/jats:italic>\u00a0=\u00a00.01) and lower CE1<\/jats:styled-content> of platelets (p<\/jats:italic>\u2009<\u20090.01) when compared with Optia device.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The knowledge of the MNC<\/jats:styled-content> and lymphocyte CE1<\/jats:styled-content> of each apheresis device used to collect cells for CAR T<\/jats:styled-content> therapy, together with the goal of the number of cells required, is essential to define the volume to be processed and to ensure the success of the collection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13394","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T18:39:16Z","timestamp":1671043156000},"page":"217-222","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of cell collection efficiency in non\u2010mobilized adult donors for autologous chimeric antigen receptor T\u2010cell manufacturing"],"prefix":"10.1111","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4576-0540","authenticated-orcid":false,"given":"Juan A.","family":"Pi\u00f1eyroa","sequence":"first","affiliation":[{"name":"Apheresis & Cellular Therapy Unit, Department of Hemotherapy and Hemostasis ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5445-4508","authenticated-orcid":false,"given":"Joan","family":"Cid","sequence":"additional","affiliation":[{"name":"Apheresis & Cellular Therapy Unit, Department of Hemotherapy and Hemostasis ICMHO, Hospital Cl\u00ednic Barcelona Spain"},{"name":"IDIBAPS Barcelona Spain"},{"name":"University of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandru","family":"Vlagea","sequence":"additional","affiliation":[{"name":"Department of Immunology CDB, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria","family":"Carbass\u00e9","sequence":"additional","affiliation":[{"name":"Apheresis & Cellular Therapy Unit, Department of Hemotherapy and Hemostasis ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Henao","sequence":"additional","affiliation":[{"name":"Apheresis & Cellular Therapy Unit, Department of Hemotherapy and Hemostasis ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noem\u00ed","family":"Bailo","sequence":"additional","affiliation":[{"name":"Apheresis & Cellular Therapy Unit, Department of Hemotherapy and Hemostasis ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valent\u00edn","family":"Ortiz\u2010Maldonado","sequence":"additional","affiliation":[{"name":"IDIBAPS Barcelona Spain"},{"name":"Department of Hematology ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00faria","family":"Mart\u00ednez\u2010Cibrian","sequence":"additional","affiliation":[{"name":"Department of Hematology ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Espa\u00f1ol","sequence":"additional","affiliation":[{"name":"Department of Immunology CDB, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Delgado","sequence":"additional","affiliation":[{"name":"IDIBAPS Barcelona Spain"},{"name":"University of Barcelona Barcelona Spain"},{"name":"Department of Hematology ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro","family":"Urbano\u2010Ispizua","sequence":"additional","affiliation":[{"name":"IDIBAPS Barcelona Spain"},{"name":"University of Barcelona Barcelona Spain"},{"name":"Department of Hematology ICMHO, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2593-833X","authenticated-orcid":false,"given":"Miquel","family":"Lozano","sequence":"additional","affiliation":[{"name":"Apheresis & Cellular Therapy Unit, Department of Hemotherapy and Hemostasis ICMHO, Hospital Cl\u00ednic Barcelona Spain"},{"name":"IDIBAPS Barcelona Spain"},{"name":"University of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21705"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.03.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15287"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14429"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1994.341095026976.x"},{"key":"e_1_2_8_7_1","first-page":"715","volume-title":"Apheresis: principles and practice","author":"McLeod BC","year":"2010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14502"},{"key":"e_1_2_8_9_1","first-page":"224","article-title":"Prediction of blood volume in normal human adults","volume":"51","author":"Nadler 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