{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T20:49:47Z","timestamp":1757450987671,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Corrected count increment (CCI) measurements monitor the effectiveness of platelet transfusions in haemato\u2010oncology, but they usually fail in patients undergoing cardiac surgery. We investigated whether polymerase chain reaction (PCR) of mitochondrial single\u2010nucleotide polymorphisms (SNPs) is able to monitor the survival of transfused platelets in these patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Leukocyte\u2010free, platelet\u2010rich plasma was prepared from patients' blood to measure platelet counts based on patient\u2010\/donor\u2010specific SNPs by digital PCR after DNA extraction. Platelet counts in samples from patients with severe thrombocytopenia were analysed by both PCR and flow cytometry. Ten patients undergoing cardiac surgery with the use of heart lung machine and without overt bleeding received a single apheresis platelet concentrate because of either dual platelet inhibition during a non\u2010elective intervention or a complex procedure. Blood samples were collected at nine defined intervals (0\u2013120\u2009h) post transfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The digital PCR of the seven SNPs reliably quantified levels \u22650.6\u2009G\/L platelets, in good agreement with flow cytometry and without interference by other SNPs or by platelet activation. A mean 24\u2010h CCI of 11.8 (range: 5.6\u201319.8) and a mean 120\u2010h area under the curve (AUC) of 1386 (915\u20131821) hxG\/L were observed for the transfused platelets. The mean AUC of 14,103 (3415\u201327,305) hxG\/L for the patients' endogenous platelets indicates that transfused platelets represented only 11% (5\u201325) of the total platelet counts during 120\u2009h post transfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PCR of mitochondrial SNPs offers a tool to assess the survival of platelets from apheresis concentrates in cardiac surgery patients to facilitate the implementation of improved transfusion strategies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13422","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T10:01:13Z","timestamp":1678701673000},"page":"384-391","source":"Crossref","is-referenced-by-count":4,"title":["Digital polymerase chain reaction to monitor platelet transfusions in cardiac surgery patients"],"prefix":"10.1111","volume":"118","author":[{"given":"Khaldoun","family":"Ali","sequence":"first","affiliation":[{"name":"Clinic for Cardiac, Thoracic and Vascular Surgery Klinikum Braunschweig GmbH  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5417-6787","authenticated-orcid":false,"given":"Thomas H.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute for Clinical Transfusion Medicine, Klinikum Braunschweig GmbH  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk S. P.","family":"Garritsen","sequence":"additional","affiliation":[{"name":"Institute for Clinical Transfusion Medicine, Klinikum Braunschweig GmbH  Braunschweig Germany"},{"name":"Department of Atmospheric Pressure Plasma Processes Fraunhofer Institute for Surface Engineering and Thin Films IST  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Harringer","sequence":"additional","affiliation":[{"name":"Clinic for Cardiac, Thoracic and Vascular Surgery Klinikum Braunschweig GmbH  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Doescher","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Blood Donor Service NSTOB  Oldenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0904084"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.7326\/M14-1589"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2021.07.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.76.1734"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12576"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15990"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-393165"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2018.08.007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2018.291278"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03261.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05352.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12110"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02662.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-08-2724"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13038"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqy052"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14868"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15779"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15637"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16747"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000003550"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15511"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.768458"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.13422","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.13422","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.13422","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T12:12:52Z","timestamp":1692447172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.13422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,13]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/vox.13422"],"URL":"https:\/\/doi.org\/10.1111\/vox.13422","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"type":"print","value":"0042-9007"},{"type":"electronic","value":"1423-0410"}],"subject":[],"published":{"date-parts":[[2023,3,13]]}}}