{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:35Z","timestamp":1747188455292,"version":"3.40.5"},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Large clinical trials have demonstrated that some patient groups with hypoproliferative thrombocytopenia benefit from prophylactic platelet transfusions, while in others, a therapeutic transfusion regimen might be sufficient. The remaining capacity to generate endogenous platelets might be helpful to select the platelet transfusion regimen. We assessed whether the recently described method of digital droplet polymerase chain reaction (PCR) can be used to assess the endogenous platelet levels in two groups of patients undergoing high\u2010dose chemotherapy with autologous stem cell transplantation (ASCT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Multiple myeloma (<jats:italic>n<\/jats:italic>\u2009=\u200922) patients received high\u2010dose melphalan alone (HDMA); lymphoma patients (<jats:italic>n<\/jats:italic>\u2009=\u200915) received BEAM or TEAM (B\/TEAM) conditioning. Patients with a total platelet count &lt;10\u2009G\/L received prophylactic apheresis platelet concentrates. Daily endogenous platelet counts were measured by digital droplet PCR for at least 10\u2009days post\u2010ASCT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Post\u2010transplantation B\/TEAM patients received their first platelet transfusion on average 3\u2009days earlier than HDMA patients (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and required about twofold more platelet concentrates (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). The endogenous platelet count fell \u22645\u2009G\/L for a median of 115\u2009h (91\u2013159; 95% confidence interval) in B\/TEAM\u2010treated patients compared to 12.6\u2009h (0\u201324) (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) in HDMA\u2010treated patients. Multivariate analysis confirmed this profound effect of the high\u2010dose regimen (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). The CD\u201034<jats:sup>+<\/jats:sup>\u2010cell dose in the graft was inversely correlated with the intensity of endogenous thrombocytopenia in B\/TEAM\u2010treated patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Monitoring endogenous platelet counts detects the direct effects of myelosuppressive chemotherapies on platelet regeneration. This approach may help to develop a platelet transfusion regimen tailored to specific patient groups.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13445","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T05:54:30Z","timestamp":1684821270000},"page":"559-566","source":"Crossref","is-referenced-by-count":0,"title":["Intensity of endogenous thrombocytopenia after autologous stem cell transplantation in patients prophylactically transfused with platelets"],"prefix":"10.1111","volume":"118","author":[{"given":"Andreas","family":"Vo\u00df","sequence":"first","affiliation":[{"name":"Department of Oncology and Haematology University Hospital at Klinikum Oldenburg  Oldenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Doescher","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Blood Donor Service NSTOB  Oldenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Hermann","family":"Kapels","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Blood Donor Service NSTOB  Oldenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5858-5097","authenticated-orcid":false,"given":"Axel","family":"Seltsam","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Blood Donor Service NSTOB  Oldenburg Germany"},{"name":"Bavarian Red Cross Blood Service  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8343-7336","authenticated-orcid":false,"given":"Andreas","family":"Greinacher","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine University Medicine Greifswald  Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Metzner","sequence":"additional","affiliation":[{"name":"Department of Oncology and Haematology University Hospital at Klinikum Oldenburg  Oldenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5417-6787","authenticated-orcid":false,"given":"Thomas H.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Blood Donor Service NSTOB  Oldenburg Germany"},{"name":"Department of Clinical Transfusion Medicine Metropolitan Hospital  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16545"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60689-8"},{"key":"e_1_2_9_4_1","first-page":"809","article-title":"Platelet transfusion in hematology, oncology and surgery","volume":"111","author":"Wandt H","year":"2014","journal-title":"Dtsch Arztebl Int"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1212772"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2010.10.012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16657"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.5.1105.1105"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16167"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13422"},{"key":"e_1_2_9_11_1","first-page":"1086","article-title":"Efficacy of UVC\u2010treated, pathogen\u2010reduced platelets versus untreated platelets: a randomized controlled non\u2010inferiority trial","volume":"106","author":"Brixner V","year":"2021","journal-title":"Haematologica"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113677"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-01-757930"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12929"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.118075"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16126"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703365"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2021.279512"}],"container-title":["Vox 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