{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T03:39:23Z","timestamp":1781840363192,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T00:00:00Z","timestamp":1688428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Extracorporeal photopheresis (ECP) is a widespread cellular therapy for graft\u2010versus\u2010host disease, autoimmune diseases and S\u00e9zary disease. One of the main effects of ECP is the apoptosis of leukocytes, but the therapeutic mechanisms are not completely known. The aim of this study was to investigate the effects on red blood cells, platelets and the induction of reactive oxygen species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We used human cells from healthy blood donors to simulate in vitro the composition in an apheresis bag. Cells were treated with 8\u2010methoxypsoralen (8\u2010MOP) and UVA. Red blood cell stability, platelet activity and induction of reactive oxygen species were analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 8\u2010MOP and UVA treatment, the red blood cells showed high cell integrity with low levels of eryptosis and no increase of free haemoglobin or red blood cell distribution width (RDW). Red blood cell immune\u2010associated antigens CD59 and CD147 were hardly affected by the treatment. Platelet glycoproteins CD41, CD62P and CD63 indicated strong platelet activation after 8\u2010MOP and UVA treatment. Reactive oxygen species were slightly but not significantly induced by the treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The effect of the ECP therapy is probably not exclusively mediated by leukocytes. Platelet activation is another striking effect caused by the treatment of the apheresis product with 8\u2010MOP\/UVA. However, since we could hardly identify any evidence for eryptosis or haemolysis, it is unlikely that red blood cell eryptosis is part of the therapeutic mechanism. Further research on this topic seems to be promising.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13489","type":"journal-article","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T08:24:54Z","timestamp":1688459094000},"page":"775-782","source":"Crossref","is-referenced-by-count":5,"title":["Extracorporeal photopheresis and the cellular mechanisms: Effects of 8\u2010methoxypsoralen and <scp>UVA<\/scp> treatment on red blood cells, platelets and reactive oxygen species"],"prefix":"10.1111","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5133-1166","authenticated-orcid":false,"given":"Holger","family":"Budde","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Mohr","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Bogeski","sequence":"additional","affiliation":[{"name":"Molecular Physiology Division Institute of Cardiovascular Physiology, University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Riggert","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias J.","family":"Legler","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00236"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000508169"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21367"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2015.02.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20274"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.14333"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1912274"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0b013e32832ce943"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21957"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2006.05.004"},{"key":"e_1_2_8_12_1","first-page":"1","article-title":"In 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