{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:16Z","timestamp":1787016196656,"version":"build-2736575974"},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>In Japan, the prevalence of haptoglobin deficiency is approximately 1 in 4000. Haptoglobin\u2010deficient individuals may produce anti\u2010haptoglobin from allo\u2010immunization, leading to serious transfusion reactions. Therefore, implementation of a consistent supply of haptoglobin\u2010deficient fresh frozen plasma is crucial. We developed a novel reagent to facilitate large\u2010scale identification of haptoglobin\u2010deficient individuals as potential donors of plasma products.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We established mouse monoclonal anti\u2010haptoglobin\u2010producing cell lines (three clones) using the hybridoma method by immunizing mice with the haptoglobin protein. Purified antibodies were conjugated with carboxylate\u2010modified polystyrene latex beads and used for haptoglobin measurements by the latex agglutination method using an automatic analyser (LABOSPECT008). Samples with low protein concentrations were re\u2010examined by enzyme\u2010linked immunosorbent assay to confirm the results. Additionally, the\n                      <jats:italic>haptoglobin<\/jats:italic>\n                      gene was amplified by polymerase chain reaction to confirm the\n                      <jats:italic>haptoglobin<\/jats:italic>\n                      deletion allele (\n                      <jats:italic>Hp<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>del<\/jats:italic>\n                      <\/jats:sup>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      From February to October 2022, 7476 blood donor samples were screened. Two haptoglobin\u2010deficient and 21 low\u2010haptoglobin\u2010expressing individuals were identified. Two haptoglobin\u2010deficient donors were found homozygous for\n                      <jats:italic>Hp<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>del<\/jats:italic>\n                      <\/jats:sup>\n                      , and 19 (90%) of the 21 low\u2010haptoglobin\u2010expressing individuals were heterozygous for\n                      <jats:italic>Hp<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>del<\/jats:italic>\n                      <\/jats:sup>\n                      , which includes the first reported case of heterozygous\n                      <jats:italic>Hp<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>del<\/jats:italic>\n                      <\/jats:sup>\n                      <jats:italic>\/Hp<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>Johnson<\/jats:italic>\n                      <\/jats:sup>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We developed a new reagent for the detection of haptoglobin deficiency, which is automatable and inexpensive and appears useful for large\u2010scale screening of blood donors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.13543","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T23:15:48Z","timestamp":1696547748000},"page":"1109-1114","source":"Crossref","is-referenced-by-count":3,"title":["A novel reagent for the screening of haptoglobin\u2010deficient blood donors"],"prefix":"10.1111","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-257X","authenticated-orcid":false,"given":"Naoko","family":"Watanabe\u2010Okochi","sequence":"first","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Sato","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Okuyama","sequence":"additional","affiliation":[{"name":"Fujikura Kasei Co., Ltd  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Go","family":"Tomiyoshi","sequence":"additional","affiliation":[{"name":"Fujikura Kasei Co., Ltd  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Kitsukawa","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Anazawa","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashige","family":"Shimoyamada","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Onodera","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Uchikawa","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7619-6500","authenticated-orcid":false,"given":"Nelson\u2010Hirokazu","family":"Tsuno","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Muroi","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo 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