{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T20:23:04Z","timestamp":1783714984781,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Deficiencies of protein C (PC) or protein S (PS) are rare diseases, characterized by mutations in the PC or PS genes, which encode plasma serine proteases with anti\u2010coagulant activity. Severe PC or PS deficiencies manifest in early life as neonatal purpura fulminans, a life\u2010threatening heamorrhagic condition requiring immediate treatment. First\u2010line treatment involves replacement therapy, followed by maintenance with anti\u2010coagulants. Replacement therapy with specific protein concentrates is currently only limited to PC, and therefore, a PC\u2009+\u2009PS concentrate represents a useful addition to therapeutic options, particularly for severe PS deficiency. Further, the production of a PC\u2009+\u2009PS concentrate from unused plasma fractionation intermediates would impact favourably on manufacturing costs, and consequently therapy prices for patients and health systems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Several chromatographic runs were performed on the same unused plasma fractionation intermediates using different supports to obtain a PC\/PS concentrate. The best chromatographic mediums were chosen, in terms of specific activity and recovery. A full process of purification including virus inactivation\/removal and lyophilization steps was set up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The final freeze\u2010dried product had a mean PC concentration of 47.75\u2009IU\/mL with 11% of PS, and a mean specific activity of 202.5\u2009IU\/mg protein, corresponding to over 12,000\u2010fold purification from plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The development of a novel concentrated PC\/PS mixture obtained from a waste fraction of other commercial products could be used for its potential therapeutic role in the management of neonatal purpura fulminans pathology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13567","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T02:29:39Z","timestamp":1701224979000},"page":"193-202","source":"Crossref","is-referenced-by-count":3,"title":["New plasma protein <scp>C<\/scp> and protein 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