{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T06:28:08Z","timestamp":1751956088134,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T00:00:00Z","timestamp":1719705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The detection of treponemal antibodies, which are used to make a diagnosis of syphilis, is important both for diagnostic purposes and as a mandatory blood donor test in most countries. We evaluated the feasibility of using Kode Technology to make syphilis peptide red cell kodecytes for use in column agglutination serologic platforms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Candidate Kode Technology function\u2010spacer\u2010lipid (FSL) constructs were made for the <jats:italic>Treponema pallidum<\/jats:italic> lipoprotein (TmpA) of <jats:italic>T. pallidum<\/jats:italic>, using the peptide and FSL selection algorithms, and then used to make kodecytes. Developmental kodecytes were evaluated against a large range of syphilis antibody reactive and non\u2010reactive samples in column agglutination platforms and compared against established methodologies. Overall, 150 reactive and 2072 non\u2010reactive Syphicheck assay (a modified <jats:italic>T. pallidum<\/jats:italic> particle agglutination) blood donor samples were used to evaluate the agreement rate of the developed kodecyte assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>From three FSL\u2010peptide candidate constructs, one was found to be the most suitable for diagnostics. Of 150 Syphicheck assay reactive samples, 146 were TmpA\u2013kodecyte reactive (97.3% agreement), compared with 58.0% with the rapid plasmin reagin (RPR) assay for the same samples. Against the 2072 expected syphilis non\u2010reactive samples the agreement rate for TmpA\u2013kodecytes was 98.8%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TmpA\u2013kodecytes are viable for use as cost\u2010effective serologic reagent red cells for the detection of treponemal antibodies to diagnose syphilis with a high level of specificity in blood centres. This kodecyte methodology also potentially allows for introduction of the reverse\u2010algorithm testing into low\u2010volume laboratories, by utilizing existing transfusion laboratory infrastructure.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13628","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T06:00:20Z","timestamp":1719813620000},"page":"821-826","source":"Crossref","is-referenced-by-count":4,"title":["A novel syphilis <i>Treponema pallidum<\/i> lipoprotein peptide antigen diagnostic assay using red cell kodecytes in routine blood centre column agglutination testing platforms"],"prefix":"10.1111","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2094-6429","authenticated-orcid":false,"given":"Suvro Sankha","family":"Datta","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine Tata Medical Center  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radhika","family":"Nagappan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Auckland City Hospital  Auckland New Zealand"},{"name":"Kode Technology Laboratory, School of Engineering, Computer and Mathematical Sciences, Faculty of Design and Creative Technologies Auckland University of Technology  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Durba","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Tata Medical Center  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debapriya","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Tata Medical Center  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaushik","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Tata Medical Center  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradip Kumar","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Tata Medical Center  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Tuzikov","sequence":"additional","affiliation":[{"name":"Kode Biotech Limited  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolai V.","family":"Bovin","sequence":"additional","affiliation":[{"name":"Kode Biotech Limited  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Henry","sequence":"additional","affiliation":[{"name":"Kode Technology Laboratory, School of Engineering, Computer and Mathematical Sciences, Faculty of Design and Creative Technologies Auckland University of Technology  Auckland New Zealand"},{"name":"Kode Biotech Limited  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1901593"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02851.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17707"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13554"},{"key":"e_1_2_9_6_1","first-page":"197","article-title":"Syphilis testing in blood donors: an update","volume":"13","author":"Kaur G","year":"2015","journal-title":"Blood Transfus"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001825"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40471-018-0138-z"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1436080"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa349"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02555-10"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa307"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5858\/2016-0110-CP"},{"key":"e_1_2_9_14_1","unstructured":"HenrySM TuzikovAB BovinNV.Kode technology illustrated technical manual. 1st ed. Kode Biotech.2023Available from:https:\/\/hdl.handle.net\/2292\/62953. Last accessed 10 Nov 2023."},{"key":"e_1_2_9_15_1","unstructured":"NagappanR.Kode technology peptide\u2010based kodecyte diagnostics using spirochetes and SARS CoV\u20102 as models [PhD Thesis]. Auckland University of Technology.2021Available from:https:\/\/hdl.handle.net\/10292\/14809. Last accessed 10 Nov 2023."},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16327"},{"key":"e_1_2_9_17_1","first-page":"72","article-title":"Erytra blood group analyser and Kode technology testing of SARS\u2010CoV\u20102 antibodies among convalescent patients and vaccinated individuals","volume":"3","author":"Weinstock C","year":"2022","journal-title":"Eur J Hematol"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00830-21"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa350"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21186-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.15322"},{"key":"e_1_2_9_22_1","unstructured":"Syphicheck\u2010WB.Rapid test for syphilis (modified TPHA). Available from:https:\/\/www.tulipgroup.com\/PDFs\/Rapid_Tests\/IFU\/SYPHICHECK\u2010WB\u2010(Device)\u2010IFU.pdf. Last accessed 10 Nov 2023."},{"key":"e_1_2_9_23_1","first-page":"525","article-title":"Continuous population surveillance of COVID\u201019 immunity can be provided by blood services at low cost using routine laboratory infrastructure","volume":"6","author":"Henry SM","year":"2022","journal-title":"Blood Transfus"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2006.024265"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.309_17554"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.13628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T08:50:59Z","timestamp":1723539059000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.13628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,30]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/vox.13628"],"URL":"https:\/\/doi.org\/10.1111\/vox.13628","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"type":"print","value":"0042-9007"},{"type":"electronic","value":"1423-0410"}],"subject":[],"published":{"date-parts":[[2024,6,30]]}}}