{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T21:13:02Z","timestamp":1782421982097,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The isolation of neutrophils and subsequent detection of anti\u2010human neutrophil antigens (HNA) antibodies are crucial in clinical medicine for the diagnosis of autoimmune neutropenia, neonatal alloimmune neutropenia (NAIN) and transfusion\u2010related acute lung injury (TRALI). This study reports two cases of maternal anti\u2010Fc\u2010gamma\u2010receptor\u2010IIIb (Fc\u03b3RIIIb) isoimmunization without NAIN symptoms and compares the efficiency of immunomagnetic negative selection (IMNS) with traditional dextran\/Ficoll for neutrophil isolation in HNA serological assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Investigating two cases of maternal anti\u2010Fc\u03b3RIIIb isoimmunization, neutrophils from three donors were isolated from 8\u2009mL of whole blood using IMNS and dextran\/Ficoll. Serological assays included the granulocyte agglutination and immunofluorescence test, monoclonal antibody immobilization of granulocyte antigens and the LABScreen Multi (One Lambda). IMNS and dextran\/Ficoll were compared in terms of cell yield, viability, time, cost and purity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Maternal anti\u2010Fc\u03b3RIIIb isoantibodies with <jats:italic>FCGR3B<\/jats:italic> gene deletion were detected in both cases. Newborns and fathers exhibited specific gene combinations: <jats:italic>FCGR3B*02\/FCGR3B*02<\/jats:italic> (Case 1) and <jats:italic>FCGR3B*02\/FCGR3B*03<\/jats:italic> (Case 2). IMNS outperformed dextran\/Ficoll, yielding four times more neutrophils (average neutrophil counts: 18.5\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\/\u03bcL vs. 4.5\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\/\u03bcL), efficiently removing non\u2010neutrophil cells and reducing processing time (30\u201340\u2009min vs. 70\u201390\u2009min), although it incurred a higher cost (2.7 times).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Two cases of maternal anti\u2010Fc\u03b3RIIIb isoantibodies, unrelated to NAIN, were identified. Although neutropenia has not been described in these cases, we emphasize the importance of identifying asymptomatic cases with the potential for severe neutropenia. Additionally, IMNS is introduced as a rapid, high\u2010yield, high\u2010purity neutrophil isolation technique, beneficial for serological assays detecting anti\u2010HNA antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13631","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T11:34:44Z","timestamp":1712748884000},"page":"712-719","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of maternal <scp>Fc gamma receptor IIIb<\/scp> isoantibodies using immunomagnetic negative selected neutrophils"],"prefix":"10.1111","volume":"119","author":[{"given":"Juliana Oliveira","family":"Martins","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Oncology Universidade Federal de S\u00e3o Paulo, UNIFESP  S\u00e3o Paulo 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melca Maria Oliveira","family":"Barros","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Oncology Universidade Federal de S\u00e3o Paulo, UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"de Marco","sequence":"additional","affiliation":[{"name":"Instituto de Imunogen\u00e9tica, IGEN  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Fantini","family":"Machado","sequence":"additional","affiliation":[{"name":"Instituto de Imunogen\u00e9tica, IGEN  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Orlando","family":"Bordin","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Oncology Universidade Federal de S\u00e3o Paulo, UNIFESP  S\u00e3o Paulo 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