{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T15:58:26Z","timestamp":1782230306316,"version":"3.54.5"},"reference-count":8,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The World Health Organization (WHO) African Region accounts for 94% of malaria cases globally, with variability recognized within endemic regions. To determine the detection rate of <jats:italic>Plasmodium<\/jats:italic> RNA in blood donors resident in malaria\u2010endemic areas, samples from three African countries were tested using a <jats:italic>Plasmodium<\/jats:italic> nucleic acid test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Whole blood (WB) samples collected from routine donors in Cameroon, Madagascar and Mali were shipped frozen to the United States. Samples were tested individually from WB lysates with the Procleix Plasmodium assay (transcription\u2010mediated amplification [TMA]). Reactive samples were considered either repeat reactive or initial reactive only, depending on TMA\u2010retest results. When available, matching plasma samples were tested for <jats:italic>Plasmodium<\/jats:italic> antibodies by enzyme immunoassay (EIA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Plasmodium<\/jats:italic> repeat reactivity ranged from 41% (91\/223 tested) in Cameroon to 12% (26\/216) in Mali and 1% (3\/249) in Madagascar. Initially reactive samples, where reactivity did not repeat, were identified from Cameroon (5\/223; 2%) and Mali (2\/216; 1%). The matched\u2010plasma subgroup had EIA reactivity ranging from 86% (113\/131 tested) in Cameroon to 59% (10\/17) in Mali and 27% (68\/248) in Madagascar.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Plasmodium<\/jats:italic> ribosomal RNA (rRNA) detection and antibody rates varied greatly in the three countries studied. Detection of <jats:italic>Plasmodium<\/jats:italic> rRNA can provide an additional tool to address malaria risk in blood donors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13756","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T01:29:31Z","timestamp":1730338171000},"page":"71-75","source":"Crossref","is-referenced-by-count":4,"title":["Estimated <i>Plasmodium<\/i><scp>18S ribosomal RNA<\/scp> prevalence in asymptomatic blood donors from three African countries"],"prefix":"10.1111","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7735-8420","authenticated-orcid":false,"given":"Laura","family":"Tonnetti","sequence":"first","affiliation":[{"name":"American Red Cross Scientific Affairs  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamel A.","family":"Groves","sequence":"additional","affiliation":[{"name":"American Red Cross Scientific Affairs  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna","family":"Self","sequence":"additional","affiliation":[{"name":"Grifols Diagnostic Solutions Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha C.","family":"Yadav","sequence":"additional","affiliation":[{"name":"Grifols Diagnostic Solutions Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2179-3105","authenticated-orcid":false,"given":"Claude","family":"Tayou Tagny","sequence":"additional","affiliation":[{"name":"Centre Hospitalier et Universitaire du Cameroun  Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivat A.","family":"Rakoto Alson","sequence":"additional","affiliation":[{"name":"University Hospital of Antananarivo  Antananarivo Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Livezey","sequence":"additional","affiliation":[{"name":"Grifols Diagnostic Solutions Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery M.","family":"Linnen","sequence":"additional","affiliation":[{"name":"Grifols Diagnostic Solutions Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Stramer","sequence":"additional","affiliation":[{"name":"Infectious Disease Consultant  North Potomac Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00016-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofz283"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000525414"},{"key":"e_1_2_9_5_1","unstructured":"WHO Global status report on blood safety and availability 2021. Available from:https:\/\/www.who.int\/publications\/i\/item\/9789240051683. Last accessed 15 Aug 2024."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17612"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15775"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16718"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2015.03.004"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.13756","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T23:03:59Z","timestamp":1737587039000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.13756"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,30]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/vox.13756"],"URL":"https:\/\/doi.org\/10.1111\/vox.13756","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,30]]}}}