{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:45:41Z","timestamp":1784951141042,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2025,2]]},"abstract":"Abstract<\/jats:title>Background and Objectives<\/jats:title>In the setting of tissue hypoxia, S\u2010nitrosylated haemoglobin (SNO\u2010Hb) plays crucial roles in the control of blood flow. This is associated with decreased oxygen affinity to haemoglobin and increase in tissue oxygenation. Red blood cell (RBC) transfusion is primarily performed to improve tissue oxygenation in anaemic patients. RBCs after storage undergo a variety of biochemical and functional alterations, including deficiency of nitric oxide (NO) bioactivity. However, how donor characteristics affect NO levels during RBC storage is unclear. We sought to investigate the association of blood donor age, gender and storage duration with NO and SNO\u2010Hb levels in blood units.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>Blood samples from 42 healthy younger (\u226430\u2009years) and older (\u226545\u2009years) donors were collected and stored for up to 42\u2009days. Total NO kits were used to detect total nitrite and nitrate levels in blood storage solution. SNO\u2010Hb levels in RBCs were detected and analysed by quantitative mass spectrometry.<\/jats:p><\/jats:sec>Results<\/jats:title>Total NO levels in the blood storage solution significantly increased with donor age and storage duration. Proteomic analysis revealed that RBCs from older donors, particularly older females, significantly lost SNO\u2010Hb during storage. Our findings indicate that RBCs from older donors are associated with reduced SNO\u2010Hb levels and increased NO metabolites in storage solution after \u226535\u2009days storage.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The findings suggest stored RBCs from older donors may have reduced capacity to deliver oxygen to tissues under hypoxia. A shorter shelf life may be required for storing RBCs from older donors, particularly older females.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.13768","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:04:28Z","timestamp":1732233868000},"page":"132-139","source":"Crossref","is-referenced-by-count":1,"title":["Effects of blood donor characteristics and storage on red blood cell haemoglobin \u03b2 S\u2010nitrosylation"],"prefix":"10.1111","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0409-1232","authenticated-orcid":false,"given":"Cuiping","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Jacksonville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Jacksonville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravinder J.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Jacksonville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abba C.","family":"Zubair","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Jacksonville Florida USA"},{"name":"Center for Regenerative Biotherapeutics, Mayo Clinic Jacksonville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,21]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Blood transfusion","author":"Lotterman S","year":"2022"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.9185"},{"key":"e_1_2_9_4_1","first-page":"222","article-title":"Red blood cell storage time and transfusion: current practice, concerns and future perspectives","volume":"15","author":"Garcia\u2010Roa M","year":"2017","journal-title":"Blood 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