{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:37Z","timestamp":1760142037779,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Bacterial contamination of platelets stored at room temperature poses the highest risk of infection and fatality. Our hospital\u2010based blood bank performs bacterial culture using the Bactec\u2122 FX system for all whole blood\u2013derived and apheresis units. Approximately 1\u2009mL is collected from each of three to four buffy coat whole blood\u2013derived platelet concentrates (PCs). The pooled sample is then inoculated into a single aerobic paediatric culture bottle. For apheresis units, 3 mL is inoculated per unit. Inoculation is performed at least 12\u2009h after donation or 16\u201324\u2009h after processing, when feasible. This study aims to determine the prevalence of culture\u2010positive PCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A retrospective review of the blood bank's records from April 2021 to August 2023 was carried out. The number of positive platelet cultures, identified organisms and their clinical significance were analysed. Clinical records were reviewed for any reported transfusion reactions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 5009 PCs were cultured. The rate of initial positive Bactec\u2122 FX system results of pooled samples was 1.7%, corresponding to 85 PCs. <jats:italic>Staphylococcus<\/jats:italic> species accounted for 48% of the identified organisms. Upon repeat testing from individual PCs, five\u2009units (1:1000\u2009units) were confirmed to be positive: four with <jats:italic>Micrococcus luteus<\/jats:italic> and one with Gram\u2010positive bacilli. No clinical sequelae were reported in the recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates a low platelet culture positivity rate, with the most common contaminant being <jats:italic>Staphylococcus<\/jats:italic> species. Further studies are required to evaluate the feasibility of a large\u2010volume delayed\u2010sampling testing protocol in our setting.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vox.70096","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:39:36Z","timestamp":1755131976000},"page":"1038-1043","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial screening of platelet components: Experience at a hospital\u2010based blood bank"],"prefix":"10.1111","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8649-0650","authenticated-orcid":false,"given":"Arwa Z.","family":"Al\u2010Riyami","sequence":"first","affiliation":[{"name":"Department of Hematology Sultan Qaboos University Hospital, University Medical City  Muscat Oman"},{"name":"Department of Hematology College of Medicine and Health Sciences, Sultan Qaboos University  Muscat Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Al\u2010Ruzeiqi","sequence":"additional","affiliation":[{"name":"College of Medicine &amp; Health Sciences, Sultan Qaboos University  Muscat Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Al\u2010Omairi","sequence":"additional","affiliation":[{"name":"Department of Hematology Sultan Qaboos University Hospital, University Medical City  Muscat Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Al\u2010Muharrmi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology College of Medicine &amp; Health Sciences, Sultan Qaboos University  Muscat Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-655944"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.18295\/2075-0528.1592"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2212-9"},{"key":"e_1_2_9_5_1","unstructured":"Food and Drug Administration (FDA).Fatalities Reported to FDA following blood collection and transfusion Annual Summary for Fiscal Year 2021.2021. Available from:https:\/\/www.fda.gov\/media\/172382\/download. Last accessed 15 May 2025"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000381996"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12883"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14085"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12561"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16112"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12020258"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13283"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13519"},{"key":"e_1_2_9_14_1","unstructured":"Accreditation Canada. Available from:https:\/\/accreditation.ca\/. Last accessed 12 Jul 2025"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2013.10.006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.9734\/jammr\/2023\/v35i245328"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/hematology.2022000402"},{"key":"e_1_2_9_18_1","unstructured":"Food and Drug Administration.Bacterial risk control strategies for blood collection establishments and transfusion services to enhance the safety and availability of platelets for transfusion guidance for industry.2020. Available from:https:\/\/www.fda.gov\/media\/123448\/download. 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