{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:21:45Z","timestamp":1765412505091,"version":"3.46.0"},"reference-count":11,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>The discovery of circulating fetal DNA in maternal plasma enabled non\u2010invasive prenatal testing (NIPT) for targeted anti\u2010D prophylaxis. In 2019, Ireland implemented an in\u2010house test to guide this care. Here, we report 6\u2009years of service.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Cell\u2010free fetal DNA (cffDNA) was extracted from 1000\u2010\u03bcL plasma from pregnant RhD negative women at gestation age (GA)\u2009&gt;\u200911\u2009weeks. Samples were tested in triplicate for\n                      <jats:italic>RHD<\/jats:italic>\n                      by quantitative PCR using a multiplex assay of\n                      <jats:italic>RHD<\/jats:italic>\n                      exons 7 and 10.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of the 20,099 samples tested, 58.2% were predicted RhD positive and 36.9% were predicted RhD negative. Discrepancies were seen in 52 cases: 7 (0.03%) were predicted RhD negative but cord RhD positive and 45 (0.2%) were predicted RhD positive but cord RhD negative.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This test has ensured that over 7400 women have avoided unnecessary anti\u2010D prophylaxis. There were few discrepant results, with false negatives posing a greater clinical risk due to withheld anti\u2010D, increasing immunization risk. Three were linked to pre\u2010analytical errors prompting procedural changes. Due to the success of fetal\n                      <jats:italic>RHD<\/jats:italic>\n                      screening, cord blood testing has been discontinued in some countries; a practice which could be reviewed in Ireland.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70107","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T01:05:19Z","timestamp":1757552719000},"page":"1259-1262","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of 6\u2009years of fetal\n                    <i>RHD<\/i>\n                    screening in Ireland: From implementation to practice"],"prefix":"10.1111","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1135-2824","authenticated-orcid":false,"given":"Helen","family":"Ryan","sequence":"first","affiliation":[{"name":"Blood Group Genetics Laboratory Irish Blood Transfusion Service  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1699-0629","authenticated-orcid":false,"given":"Mark","family":"Lambert","sequence":"additional","affiliation":[{"name":"Blood Group Genetics Laboratory Irish Blood Transfusion Service  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9385-9836","authenticated-orcid":false,"given":"Sorcha","family":"N\u00ed Loingsigh","sequence":"additional","affiliation":[{"name":"Blood Group Genetics Laboratory Irish Blood Transfusion Service  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1020-8983","authenticated-orcid":false,"given":"Richard","family":"Hagan","sequence":"additional","affiliation":[{"name":"Blood Group Genetics Laboratory Irish Blood Transfusion Service  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7796-2000","authenticated-orcid":false,"given":"Diarmaid","family":"O'Donghaile","sequence":"additional","affiliation":[{"name":"Blood Group Genetics Laboratory Irish Blood Transfusion Service  Dublin Ireland"},{"name":"Department of Haematology Trinity College Dublin  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12091"},{"key":"e_1_2_9_3_1","article-title":"Anti\u2010D administration after childbirth for preventing Rhesus alloimmunisation","volume":"1997","author":"Crowther C","year":"2000","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.12.3662.012k12_3662_3668"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000321347"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.13055"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.i5789"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03362.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.1.12"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4419"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000531694"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:18:47Z","timestamp":1765412327000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,10]]},"references-count":11,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/vox.70107"],"URL":"https:\/\/doi.org\/10.1111\/vox.70107","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"type":"print","value":"0042-9007"},{"type":"electronic","value":"1423-0410"}],"subject":[],"published":{"date-parts":[[2025,9,10]]}}}