{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T13:41:04Z","timestamp":1770817264708,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["75N92019D00038"],"award-info":[{"award-number":["75N92019D00038"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["75N92019D00033"],"award-info":[{"award-number":["75N92019D00033"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Arbovirus infections are a major public health concern in Brazil and an ongoing blood safety concern. Periodic outbreaks of such infections are common in the general population. This study aimed to establish the rates of dengue, chikungunya and Zika virus (DENV, CHIKV, ZIKV) RNAemia among blood donors at six public blood centres during the 2019\u20132020 and 2020\u20132021 outbreak seasons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Residual minipool samples from nucleic acid testing (NAT) screening for human immunodeficiency virus, hepatitis B virus and hepatitis C virus were further pooled to form pools of 18 donation samples and tested using a Grifols research\u2010use\u2010only triplex transcription\u2010mediated amplification assay for DENV, CHIKV and ZIKV RNA to establish the rates of RNAemia and infection incidence. We used these rates and estimated the durations of RNAemia, juxtaposed with public health reporting of cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 5,616 minipools representing 101,088 donations were tested. During both outbreak seasons, the highest rates of DENV RNAemia were observed in Ribeir\u00e3o Preto, at 122\/100,000 donations (95% confidence interval [CI]: 66\u2013224) and 100\/100,000 (95% CI: 52\u2013189), respectively, with DENV RNAemia also detected in S\u00e3o Paulo, Recife and Manaus in the 2019\/2020 season and in the latter two during the 2020\/2021 season. CHIKV RNAemia was detected in Recife and ZIKV RNAemia in Manaus during the 2020\/2021 season. The estimated numbers of DENV, CHIKV and ZIKV RNAemic components released for transfusion over the study period were 338, 22 and 6, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Surveillance for arbovirus RNAemia in blood donors is a useful adjunct to public health surveillance, particularly when surveillance systems are under strain, and has implications for transfusion safety.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70150","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T01:14:28Z","timestamp":1766020468000},"page":"196-201","source":"Crossref","is-referenced-by-count":0,"title":["Dengue, chikungunya and\n                    <scp>Zika<\/scp>\n                    virus surveillance in blood donors in\n                    <scp>Brazil<\/scp>\n                    , 2019\u20132021"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7046-7245","authenticated-orcid":false,"given":"Eduard","family":"Grebe","sequence":"first","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"},{"name":"SACEMA, Centre for Epidemic Response and Innovation (CERI) Stellenbosch University  Stellenbosch South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3217-5898","authenticated-orcid":false,"given":"Renata","family":"Buccheri","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"},{"name":"Department of Epidemiology and Biostatistics University of California, San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leilani","family":"Montalvo","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1487-0141","authenticated-orcid":false,"given":"Simone","family":"Kashima","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemocentro de Ribeir\u00e3o Preto  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Miranda","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade: Infectologia e Medicina Tropical, Faculdade de Medicina Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Milani","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mars","family":"Stone","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"},{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin","family":"Livezey","sequence":"additional","affiliation":[{"name":"Grifols Diagnostics  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligia","family":"Capuani","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia S.","family":"Alencar","sequence":"additional","affiliation":[{"name":"LIM\u201003 Laborat\u00f3rio de Medicina Laboratorial, Hospital das Cl\u00ednicas, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Amorim","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemorio  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maisa","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemope  Recife Pernambuco Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allyson Guimar\u00e3es","family":"da Costa","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hospitalar de Hematologia e Hemoterapia do Amazonas  Manaus Amazonas Brazil"},{"name":"Universidade Federal do Amazonas  Manaus Amazons 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6251-366X","authenticated-orcid":false,"given":"Brian","family":"Custer","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"},{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Recipient Epidemiology and Donor Evaluation Study\u2010IV\u2010Pediatric (REDS\u2010IV\u2010P) 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