{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T05:26:28Z","timestamp":1770787588198,"version":"3.50.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"Abstract<\/jats:title>\n \n Background and Objectives<\/jats:title>\n \n The administration of anti\u2010D immunoglobulin achieved a significant reduction in the mortality of haemolytic disease of the foetus and the newborn due to RhD sensitization. Between 30% and 40% of this group of pregnant women, carriers of an RhD\u2010negative foetus, would unnecessarily receive a high\u2010cost drug, exposing them to the risks of a blood derivative. The aim of the study was to assess the accuracy of non\u2010invasive foetal\n RhD<\/jats:italic>\n genotyping at different weeks of pregnancy, to allow it to be used to determine appropriate anti\u2010D administration.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n In this cross\u2010sectional cohort study of foetal\n RhD<\/jats:italic>\n genotyping, determined from cell\u2010free foetal DNA (cffDNA) between weeks 8 and 30 of gestation, 270 samples from RhD\u2010negative pregnant women were examined. Foetal Rh status was determined by multiplex PCR in real time, to detect targeting exons 5, 7, and 10 of the\n RHD<\/jats:italic>\n gene and the\n SRY<\/jats:italic>\n gene (a Y\u2010chromosome\u2010specific marker used to confirm male foetal sex).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The performance diagnostic of the foetal\n RhD<\/jats:italic>\n screen test offered a sensitivity of 100% (95% confidence interval [CI]: 98.03%\u2013100%), a specificity of 100% (95% CI: 96.3%\u2013100%) and an accuracy of 100%. The prevalence of RhD\u2010negative newborns in RhD\u2010negative pregnant women was 32.5%.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The results of the study reveal that the strategy of combining three\n RhD<\/jats:italic>\n exons to determine foetal RhD status is reliable, with 100% accuracy. Prenatal diagnosis showed that 32.5% of newborns were RhD negative. Using this strategy, a decrease in the use of anti\u2010D immunoglobulin is expected.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vox.70158","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T03:54:21Z","timestamp":1764647661000},"page":"189-195","source":"Crossref","is-referenced-by-count":0,"title":["Non\u2010invasive foetal\n \n RhD<\/i>\n <\/scp>\n genotyping: A strategy for rationalizing anti\u2010D immunoglobulin prophylaxis in\n RhD<\/scp>\n \u2010negative pregnant women at the Hospital Italiano de Buenos Aires"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1400-0899","authenticated-orcid":false,"given":"Diego Mario","family":"Santoro","sequence":"first","affiliation":[{"name":"Servicio de Medicina Transfusional Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2502-6729","authenticated-orcid":false,"given":"Paula","family":"Gonzalez Hermida","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Cl\u00ednica Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Virginia","family":"Gamboa","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Transfusional Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hernan Jorge","family":"Garcia Rivello","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Cl\u00ednica Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar","family":"Meller","sequence":"additional","affiliation":[{"name":"Servicio de Obstetricia Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Ota\u00f1o","sequence":"additional","affiliation":[{"name":"Servicio de Obstetricia Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horacio Antonio","family":"Aiello","sequence":"additional","affiliation":[{"name":"Servicio de Obstetricia Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo Javier","family":"Camino","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Transfusional Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1615-2327","authenticated-orcid":false,"given":"Leandro Daniel","family":"Burgos Pratx","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Transfusional Hospital Italiano Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"National Institute for Health and Care Excellence (NICE).Routine antenatal anti\u2010D prophylaxis for women who are rhesus D negative. Review of NICE technology appraisal guidance. Available from:https:\/\/www.nice.org.uk\/guidance\/ta156\/resources\/routine\u2010antenatal\u2010antid\u2010prophylaxis\u2010for\u2010women\u2010who\u2010are\u2010rhesus\u2010d\u2010negative\u2010pdf\u201082598318102725. Last accessed 21 Jul 2025."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12091"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118493595"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12275"},{"key":"e_1_2_9_6_1","first-page":"37","article-title":"Gammaglobulina anti\u2010D e inmunoprofilaxis. Historia, importancia, usos y obtenci\u00f3n","volume":"66","author":"Aguila RY","year":"2019","journal-title":"Rev Mex Patol Clin Med Lab"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199904223401602"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1999.7630175.x"},{"key":"e_1_2_9_9_1","unstructured":"NarayanS PolesD WoodE AlmusawyM AlradiM AlshurafaH et al. on behalf of the Serious Hazards of Transfusion (SHOT) Steering Group.Annual SHOT Report 2022. Available from:https:\/\/www.shotuk.org\/wp\u2010content\/uploads\/2024\/11\/annual\u2010SHOT\u2010report\u20102022.pdf. Last accessed 21 Jul 2025."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199812103392402"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2013.11.014"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12768"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12908"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10080564"},{"key":"e_1_2_9_16_1","first-page":"66","article-title":"Genotyping approach for non\u2010invasive foetal RHD detection in an admixed population","volume":"15","author":"Boggione CT","year":"2017","journal-title":"Blood Transfus"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000490156"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03297.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20440"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13172"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2011.01540.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.4238\/2011.October.26.1"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.2478\/bjmg-2013-0029"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000322959"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.06.054"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000085085"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tracli.2008.01.003"},{"key":"e_1_2_9_28_1","unstructured":"Food & Drug Administration (FDA).CBER\u2010regulated products: current shortages. Available from:https:\/\/www.fda.gov\/vaccines\u2010blood\u2010biologics\/safety\u2010availability\u2010biologics\/cber\u2010regulated\u2010products\u2010current\u2010shortages. Last accessed 21 July 2025."},{"key":"e_1_2_9_29_1","unstructured":"American Society of Health\u2010System Pharmacists (ASHP).Rho(D) immune globulin (human). Available from:https:\/\/www.ashp.org\/drug\u2010shortages\/current\u2010shortages\/drug\u2010shortage\u2010detail.aspx?id=1022. Last accessed 21 Jul 2025."},{"key":"e_1_2_9_30_1","unstructured":"Association for the Advancement of Blood & Biotherapies (AABB).Regulatory update: FDA announces resolution of WinRho RhIG shortage. Available from:https:\/\/www.aabb.org\/docs\/default\u2010source\/default\u2010document\u2010library\/resources\/association\u2010bulletins\/ab24\u201002.pdf. Last accessed 21 Jul 2025."}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70158","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70158","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T01:07:46Z","timestamp":1770772066000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/vox.70158"],"URL":"https:\/\/doi.org\/10.1111\/vox.70158","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,1]]}}}