{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:59Z","timestamp":1787016059476,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>High concentration of human platelet antigen\u20101a (HPA\u20101a) antibodies is reported to be associated with severe foetal and neonatal alloimmune thrombocytopaenia (FNAIT). The gold standard for quantification of anti\u2010HPA\u20101a antibodies is the monoclonal antibody immobilization of platelet antigen (MAIPA) assay, which is a laborious method performed in only a few reference laboratories. The aim of this study was to evaluate the performance of the commercially available bead\u2010based Luminex assay PakLx (Immucor) for quantitative measurement of anti\u2010HPA\u20101a antibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We analysed anti\u2010HPA\u20101a antibody levels in plasma samples from 42 HPA\u20101a\u2010negative women who had given birth to a child with thrombocytopaenia. Quantification of antibodies was performed with two different techniques: MAIPA analysed by spectrophotometry with results expressed in international units (IU)\/mL, and PakLx analysed in the Luminex assay with results expressed as the mean fluorescence intensity (MFI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the comparison of the two methods' ability to stratify a result as either positive or negative,\n                      <jats:styled-content style=\"fixed-case\">PakLx<\/jats:styled-content>\n                      demonstrated 97.6% agreement with the\n                      <jats:styled-content style=\"fixed-case\">MAIPA<\/jats:styled-content>\n                      assay, with positive and negative predictive values of 96.7% and 100%, respectively. The correlation of the\n                      <jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>\n                      values from\n                      <jats:styled-content style=\"fixed-case\">PakLx<\/jats:styled-content>\n                      with\n                      <jats:styled-content style=\"fixed-case\">IU<\/jats:styled-content>\n                      \/\n                      <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>\n                      in\n                      <jats:styled-content style=\"fixed-case\">MAIPA<\/jats:styled-content>\n                      assay was high, with a correlation coefficient (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>R<\/jats:italic>\n                        <jats:sup>2<\/jats:sup>\n                      <\/jats:styled-content>\n                      ) of 0.92.\n                      <jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>\n                      values were converted into semi\u2010quantitative results: high, intermediate and low levels of anti\u2010\n                      <jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content>\n                      \u20101a.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PakLx shows high agreement with the MAIPA assay and allows fast laboratory turnaround time for the determination of anti\u2010HPA\u20101a antibody levels. The result may be of predictive value in clinical assessments.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70180","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T01:34:55Z","timestamp":1770168895000},"page":"345-351","source":"Crossref","is-referenced-by-count":1,"title":["Rapid anti\u2010HPA\u20101a antibody quantification with a Luminex bead\u2010based assay: A method evaluation"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3696-355X","authenticated-orcid":false,"given":"Klara","family":"Asplund H\u00f6gelin","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology and Transfusion Medicine Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Laboratory Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Em\u00f6ke","family":"Deschmann","sequence":"additional","affiliation":[{"name":"Department of Women's and Children's Health Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Neonatal Medicine Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petter","family":"H\u00f6glund","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Transfusion Medicine Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Medicine Huddinge Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agneta","family":"Wikman","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Transfusion Medicine Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Medicine Huddinge Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2015.12.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.04026.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)91733-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1648803"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01063.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.12515"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12725"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.6.1722.1722"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2019.102705"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2016.04.001"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/aogs.70031"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-04-569541"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.htct.2024.06.004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12509"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.htct.2020.12.008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2014-1037"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2019.102711"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(25)00029-8"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2022.101038"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12945"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.02170.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12085"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13111835"}],"container-title":["Vox 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