{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T01:14:43Z","timestamp":1778894083716,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"Abstract<\/jats:title>\n \n Background and Objectives<\/jats:title>\n The European Union's ban on the use of di(2\u2010ethylhexyl) phthalate (DEHP) in medical devices will take effect in 2030. DEHP is a plasticizer in polyvinyl chloride blood bags that helps stabilize the red blood cell membrane during hypothermic red blood cell concentrate (RBCC) storage. Recent studies have shown that RBCCs have acceptable in vitro quality after storage in DEHP\u2010free containers (e.g., plasticized with di(2\u2010ethylhexyl) terephthalate [DEHT] and stored in phosphate\u2010adenine\u2010glucose\u2010guanosine\u2010saline\u2010mannitol [PAGGSM] additive solution). To complement quality data, in this study, we compared bacterial growth in RBCCs stored in either DEHT\/PAGGSM or DEHP\/saline\u2010adenine\u2010glucose\u2010mannitol (SAGM).<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Paired ABO\u2010matched whole blood units were collected into DEHT\/PAGGSM sets, pooled and split into one DEHT\/PAGGSM and one DEHP\/SAGM bag set. RBCCs were produced using a top\/bottom buffy coat process, tested for baseline in vitro quality and sterility, and spiked with\n Yersinia enterocolitica<\/jats:italic>\n ,\n Serratia liquefaciens<\/jats:italic>\n and\n Listeria monocytogenes<\/jats:italic>\n (~10\n 2<\/jats:sup>\n \u2009CFU\/mL) and\n Cutibacterium acnes<\/jats:italic>\n (~10\n 3<\/jats:sup>\n \u2009CFU\/mL) (\n N<\/jats:italic>\n \u2009=\u20093). RBCCs were stored at 1\u20136\u00b0C for 43\u2009days and sampled weekly for bacterial enumeration. Bacterial counts were compared between DEHP\/SAGM and DEHT\/PAGGSM RBCCs over 43 days of storage.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n For\n Y. enterocolitica<\/jats:italic>\n ,\n S. liquefaciens<\/jats:italic>\n and\n C. acnes<\/jats:italic>\n , no differences in survival\/growth between DEHP\/SAGM and DEHT\/PAGGSM RBCCs were observed.\n Y. enterocolitica<\/jats:italic>\n and\n S. liquefaciens<\/jats:italic>\n grew to 10\n 8<\/jats:sup>\n \u201310\n 9<\/jats:sup>\n \u2009CFU\/mL by day 14, while\n C. acnes<\/jats:italic>\n remained at 10\n 3<\/jats:sup>\n \u2009CFU\/mL until day 43.\n L. monocytogenes<\/jats:italic>\n counts declined in DEHT\/PAGGSM compared to DEHP\/SAGM RBCC on days 0\u20137, but bacterial loads were similar (~10\n 7<\/jats:sup>\n \u2009CFU\/mL) in both bags by day 43.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n These results suggest that the bacterial safety risk of RBCCs is not increased in DEHT\/PAGGSM containers.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vox.70195","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T01:57:46Z","timestamp":1770861466000},"page":"587-592","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial proliferation is comparable in red blood cell concentrates stored in\n DEHT<\/scp>\n \/\n PAGGSM<\/scp>\n and\n DEHP<\/scp>\n \/\n SAGM<\/scp>\n containers"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-4987","authenticated-orcid":false,"given":"Sandra","family":"Ramirez\u2010Arcos","sequence":"first","affiliation":[{"name":"Product & Process Development, Canadian Blood Services Ottawa Ontario Canada"},{"name":"Department of Biochemistry, Microbiology and Immunology University of Ottawa Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuntong","family":"Kou","sequence":"additional","affiliation":[{"name":"Product & Process Development, Canadian Blood Services Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dilini","family":"Kumaran","sequence":"additional","affiliation":[{"name":"Product & Process Development, Canadian Blood Services Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1433-7357","authenticated-orcid":false,"given":"Geraldine M.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Product & Process Development, Canadian Blood Services Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Howell","sequence":"additional","affiliation":[{"name":"Product & Process Development, Canadian Blood Services Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chryslain","family":"Sumian","sequence":"additional","affiliation":[{"name":"Medical Affairs, Macopharma Mouvaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Reichenberg","sequence":"additional","affiliation":[{"name":"Medical Affairs, Macopharma Mouvaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quentin","family":"Brebant","sequence":"additional","affiliation":[{"name":"Research and Development, Macopharma Mouvaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"McTaggart","sequence":"additional","affiliation":[{"name":"Product & Process Development, Canadian Blood Services Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.0c01964"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401344"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/58.2.377"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13446"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.70087"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14583"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12982"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.70151"},{"key":"e_1_2_9_10_1","series-title":"StatPearls","volume-title":"Infectious complications of blood transfusion","author":"Buerger CS","year":"2025"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2010.06.079"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2010.240"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12782"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13057"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2008.01097.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/529143"},{"key":"e_1_2_9_17_1","volume-title":"Bacterial contamination in \u201cpractical transfusion medicine\u201d","author":"Ramirez\u2010Arcos S","year":"2022"},{"key":"e_1_2_9_18_1","volume-title":"CAN\/CSA\u2010Z902\u201325. Blood and Blood Components","author":"Canadian Standards Association","year":"2025"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(03)00226-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13344"},{"key":"e_1_2_9_21_1","unstructured":"Circular of Information for the use of human blood components. Red blood cells leukocytes reduced. Available from:https:\/\/www.blood.ca\/sites\/default\/files\/IM-00005_Rev_1.pdf. Last accessed 18 Dec 2025."}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70195","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70195","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70195","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:47:55Z","timestamp":1778892475000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,11]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/vox.70195"],"URL":"https:\/\/doi.org\/10.1111\/vox.70195","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,11]]}}}