{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T01:13:33Z","timestamp":1778894013722,"version":"3.51.4"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"Abstract<\/jats:title>\n \n Background and Objectives<\/jats:title>\n Platelet antibody screening and identification are crucial and challenging. MacKay Memorial Hospital has been dedicated to this for nearly four decades. We reviewed our experience with platelet antibody detection methods and prevalence, and presented a possible case of post\u2010transfusion purpura caused by anti\u2010CD36.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n We analyzed platelet antibody screening results from 1988 to 2024. We used solid phase red cell adherence test (SPRCA) from 1988 to 2018 (9268 samples), and parallel SPRCA\/enzyme\u2010linked immunosorbent assay (ELISA) from 2019 to 2024 (2037 samples). Positive results are further identified through flow cytometry, monoclonal antibody immobilization of platelet antigens (MAIPA), or in\u2010house platelet antigen panels. We also studied a possible case of post\u2010transfusion purpura (PTP) caused by anti\u2010CD36. A 79\u2010year\u2010old woman was treated with numerous platelet transfusions that were ineffective, with generalized purpura appearing a few days later following massive transfusion.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n From 1988\u20132018, 2800 of 9268 samples (30.2%) were positive. Six cases of neonatal alloimmune thrombocytopenia (NAITP) were identified (two anti\u2010HPA\u20103a, two anti\u2010HPA\u20105a, and two anti\u2010CD36). From 2019\u20132024, 778 of 2037 samples (38.19%) were positive. Specific antibodies were identified in eight cases, and six of them were anti\u2010CD36. Introduction of ELISA resulted in a 15\u2010fold detection rate of anti\u2010CD36 (0.02% to 0.29%), suggesting previous underestimation. In the possible PTP case, massive transfusion was started on Day 8 of hospitalization, purpura appeared on Day 15, anti\u2010CD36 was identified on Day 21, and CD36\u2010negative platelet transfusion was initiated on the following day. However, the patient eventually died of multiorgan failure on Day 29.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The prevalence of anti\u2010CD36 antibody in the Taiwanese population was previously underestimated. Clinicians should be aware that not only human platelet antigen (HPA) but CD36 should also be considered when platelet refractoriness persists despite HLA\u2010matched transfusion, as anti\u2010CD36 may cause serious complications.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vox.70200","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T02:08:58Z","timestamp":1771380538000},"page":"654-659","source":"Crossref","is-referenced-by-count":0,"title":["Studies of platelet antibodies at\n MacKay<\/scp>\n Memorial Hospital in Taiwan: Methods, case reviews and a possible case of post\u2010transfusion purpura"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6449-6639","authenticated-orcid":false,"given":"Chiang","family":"Chen\u2010Yu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Blood Bank MacKay Memorial Hospital Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Hsiao\u2010Lin","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Blood Bank MacKay Memorial Hospital Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan","family":"Yung\u2010Shu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Blood Bank MacKay Memorial Hospital Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5536-5942","authenticated-orcid":false,"given":"Cheng","family":"Wern\u2010Cherng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Blood Bank MacKay Memorial Hospital Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7484-0251","authenticated-orcid":false,"given":"Lin","family":"Marie","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Blood Bank MacKay Memorial Hospital Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/90.1.63"},{"key":"e_1_2_12_3_1","first-page":"343","article-title":"Platelet immunology. ELISA for detection of platelet antibodies, platelet\u2010specific antigens and platelet glycoproteins","volume":"39","author":"Taaning E","year":"1992","journal-title":"Dan Med Bull"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1993.33293158049.x"},{"key":"e_1_2_12_5_1","first-page":"229","volume-title":"Transfusion medicine (ZH\u2010TW). Platelet and granulocyte antigen system","author":"Shyh\u2010Chyi L","year":"2023"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1995.tb00369.x"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2004.00537.x"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12120"},{"key":"e_1_2_12_9_1","first-page":"1265","volume-title":"Tietz textbook of laboratory medicine","author":"Poston JN","year":"2023"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14369"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0161968"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2023.866407"},{"key":"e_1_2_12_13_1","first-page":"996","volume-title":"Henry's clinical diagnosis and management by laboratory methods","author":"Bray R","year":"2022"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70200","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70200","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70200","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:47:27Z","timestamp":1778892447000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70200"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,17]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/vox.70200"],"URL":"https:\/\/doi.org\/10.1111\/vox.70200","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,17]]}}}