{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T01:00:37Z","timestamp":1781139637710,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Hypoxic\/hypocapnic (HH) treatment and storage conditions have been shown to reduce oxidative stress and improve red blood cell (RBC) quality. This study aimed to validate a good manufacturing practice\u00a0HH RBC product for obtaining the licence for routine use in patients, by comparison with normoxic RBCs both without irradiation and after gamma irradiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Forty\u2010four units of leucoreduced red cell concentrates (LR\u2010RCCs) were prepared from 500\u2009mL citrate\u2013phosphate\u2013dextrose (CPD)\/phosphate\u2010adenine\u2010glucose\u2010guanosine\u2010saline\u2010mannitol (PAGGSM) whole\u2010blood donations. Paired units of ABO\u2010matched LR\u2010RCCs were pooled and split into equal aliquots. Twenty\u2010two units (control) were maintained at room temperature for 3\u2009h, while 22\u2009units underwent HH treatment for 3\u2009h using the Hemanext ONE\u00ae system. Eleven control and 11 HH LR\u2010RCCs were assigned to the non\u2010irradiated arm, and the remaining 22\u2009units (11 control, 11 HH) were irradiated on Day 14. In vitro RBC quality was measured until Day 42 (non\u2010irradiated) and Day 28 (irradiated on Day 14).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The HH product was comparable to controls in haemoglobin (Hgb) content, haematocrit, potassium and haemolysis, both without irradiation over 42\u2009days and after irradiation over 28\u2009days. HH RBCs contained 1.2\u2010fold higher adenosine triphosphate (ATP) and up to 6.2\u2010fold higher 2,3\u2010diphosphoglycerate (2,3\u2010DPG) levels. Lactate was 25% increased, and glucose levels were up to 30% decreased compared with normoxic RBCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HH storage enhanced RBC ATP and 2,3\u2010DPG levels and yielded a product fulfilling RBC quality criteria for the other parameters. These findings support its clinical use for optimizing RBC transfusion quality, particularly for irradiated units.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70218","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:14:39Z","timestamp":1773004479000},"page":"801-808","source":"Crossref","is-referenced-by-count":0,"title":["Hypoxic\u2010hypocapnic red blood cells in\n                    <scp>PAGGSM<\/scp>\n                    additive solution before and after gamma irradiation show improved metabolism"],"prefix":"10.1111","volume":"121","author":[{"given":"Soroth","family":"Chey","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine, University Hospital Leipzig, Medical Faculty Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Maier","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, University Hospital Leipzig, Medical Faculty Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Sowemimo\u2010Coker","sequence":"additional","affiliation":[{"name":"Hemanext Inc.  Lexington Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5895-4259","authenticated-orcid":false,"given":"Reinhard","family":"Henschler","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, University Hospital Leipzig, Medical Faculty Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2010.05.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/anae.12891"},{"key":"e_1_2_9_4_1","first-page":"27","article-title":"Red blood cell storage lesion: causes and potential clinical consequences","volume":"27","author":"Yoshida T","year":"2019","journal-title":"Blood 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