{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T01:00:50Z","timestamp":1781139650764,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP24ym0126154"],"award-info":[{"award-number":["JP24ym0126154"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Haemoglobin\u2010based oxygen carriers (HBOCs) are intended to treat major bleeding, particularly in emergencies. While haemoglobin vesicles (HbVs)\u2014liposome\u2010encapsulated haemoglobin with a long shelf life\u2014and other HBOCs can cause colour\u2010related interference in chemistry testing, their effect on standardized pretransfusion assays remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We evaluated the impact of HbVs in blood on routine pretransfusion testing, including ABO blood group\/Rhesus D (RhD) typing, antibody screening and cross\u2010matching, using tube and column agglutination techniques on samples collected from 10 healthy individuals and one patient with anti\u2010E antibodies. Whole blood was mixed with HbVs at 9:1 ratio for each sample.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Plasma treated with HbVs developed a dark\u2010red colouration, which interfered with the agglutination assessment. Manual replacement or automated washing of plasma containing HbVs effectively restored test clarity without compromising the accuracy of tube\u2010based assays for reverse ABO\/RhD typing, antibody screening or major cross\u2010matching. In contrast, the washing process could not be applied to column\u2010based techniques. No interference was observed between the HbVs and commonly used reagents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Tube\u2010based pretransfusion testing with manual replacement or automated washing procedures is recommended for accurate compatibility assessment of blood samples treated with HbVs. However, further validation is required for broader clinical application.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70225","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:53:44Z","timestamp":1772981624000},"page":"878-885","source":"Crossref","is-referenced-by-count":0,"title":["Impact of haemoglobin vesicles on pretransfusion testing and mitigation strategies"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1490-7150","authenticated-orcid":false,"given":"Kazuto","family":"Omae","sequence":"first","affiliation":[{"name":"Department of Blood Transfusion Medicine Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0523-7931","authenticated-orcid":false,"given":"Kazuya","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Medicine Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0627-297X","authenticated-orcid":false,"given":"Hiroaki","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Medicine Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2012-1377","authenticated-orcid":false,"given":"Ayumi","family":"Taniyama","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Medicine Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4682-0348","authenticated-orcid":false,"given":"Mayumi","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Medicine Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0681-3032","authenticated-orcid":false,"given":"Hiromi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Chemistry Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7243-3126","authenticated-orcid":false,"given":"Masanori","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Medicine Nara Medical University  Kashihara Japan"},{"name":"Department of Hematology Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/5549655"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474086.2023.2209716"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.26719\/2018.24.8.778"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bja.2021.07.027"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26199775"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17855"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.299.19.jrv80007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000001044"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318173a93f"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c00424"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/10731199.2011.637929"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.09.010"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.14033"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022007977"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2019.07.005"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdg374"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/43.1.39"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14146"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.1999.011"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2003.036"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-229"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1990.30290162894.x"},{"key":"e_1_2_9_24_1","volume-title":"Technical manual","author":"AABB","year":"2023"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.130328"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb8010010"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2008.10.023"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2025-110183"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-6247.95051"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2018-014"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.htct.2023.07.011"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70225","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:22:53Z","timestamp":1781137373000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,25]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/vox.70225"],"URL":"https:\/\/doi.org\/10.1111\/vox.70225","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,25]]}}}