{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:11:23Z","timestamp":1793383883603,"version":"4.5.0"},"reference-count":12,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102024\u201000353171"],"award-info":[{"award-number":["RS\u20102024\u201000353171"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Daratumumab (DARA), an anti\u2010CD38 monoclonal antibody used in the treatment of multiple myeloma, frequently causes false\u2010positive panreactivity in antibody screening tests. The conventional approach to resolve this interference involves treating reagent red blood cells (RBCs) with 0.2\u2009M dithiothreitol (DTT). However, this method is labour\u2010intensive, may fail to detect clinically significant antibodies and can yield inconsistent results. We aimed to assess the effectiveness of a commercial reagent containing anti\u2010CD38 Fab fragments as an alternative for mitigating DARA\u2010related interference.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We retrospectively analysed 22 blood samples from 17 patients. Antibody screening was performed using both DTT\u2010treated and DaraEx\u2010treated reagent RBCs. Clinical and laboratory data were reviewed. Additionally, patient samples were spiked with antibodies to evaluate antibody detection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All samples showed false\u2010positive panreactivity after DARA treatment. Nineteen samples were negative using both DTT\u2010 and DaraEx\u2010treated cells. Three samples remained reactive with DTT\u2010treated cells but turned negative with DaraEx. These cases were associated with lower serum albumin, impaired renal function and elevated monoclonal protein levels. DaraEx\u2010treated cells retained reactivity with anti\u2010E and anti\u2010D antibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DaraEx consistently and completely removed DARA\u2010related interference, including in cases where DTT was ineffective. It represents a safe and efficient alternative to DTT for pre\u2010transfusion antibody screening.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70251","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T01:52:43Z","timestamp":1773885163000},"page":"1082-1086","source":"Crossref","is-referenced-by-count":2,"title":["Effective resolution of daratumumab\u2010induced false\u2010positive antibody screening with commercial reagents"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-0488","authenticated-orcid":false,"given":"Han Joo","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1440-057X","authenticated-orcid":false,"given":"Suk Won","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5197-6340","authenticated-orcid":false,"given":"Yousun","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Kangdong Sacred Heart Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0574-9200","authenticated-orcid":false,"given":"Hyungsuk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3201-5728","authenticated-orcid":false,"given":"Sang\u2010Hyun","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6728-4640","authenticated-orcid":false,"given":"Heung\u2010Bum","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9781-0928","authenticated-orcid":false,"given":"Dae\u2010Hyun","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-016-0283-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13789"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13150"},{"key":"e_1_2_9_5_1","unstructured":"Mitigating the anti\u2010CD38 interference with serologic testing. AABB. Available from:https:\/\/www.aabb.org\/news-resources\/resources\/association-bulletins. Last accessed 5 Aug 2024."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-015"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14244"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13069"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12653"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17253"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-023-01240-8"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2019-131345"},{"key":"e_1_2_9_13_1","first-page":"243","article-title":"The importance of removing interference caused by Darzalex\u00ae (daratumumab) in pre\u2010transfusion testing","volume":"13","author":"Dilberovi\u0107 A","year":"2024","journal-title":"J Health Sci"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70251","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:29:20Z","timestamp":1783902560000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":12,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/vox.70251"],"URL":"https:\/\/doi.org\/10.1111\/vox.70251","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]}}}