{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:11:13Z","timestamp":1788383473333,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Daratumumab, a therapeutic human anti\u2010CD38 monoclonal antibody, improves multiple myeloma outcomes but interferes with pre\u2010transfusion testing by binding CD38 on reagent red blood cells (RBCs), potentially masking clinically significant alloantibodies. This study aimed to evaluate the effectiveness of a novel high\u2010affinity anti\u2010idiotypic anti\u2010daratumumab reagent in neutralizing daratumumab interference while preserving RBC alloantibody detection and ensuring compatibility with routine transfusion laboratory workflows.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A non\u2010interventional, multicentre study across 28 transfusion laboratories in 15 countries evaluated a novel anti\u2010idiotypic anti\u2010daratumumab reagent (DaraClear). In total, 443 daratumumab\u2010containing plasma samples and 197 RBC alloantibody\u2013containing samples were tested. All samples were initially tested at 10% (v\/v), with stepwise escalation to 20% and 30% only if neutralization was incomplete. Neutralization efficiency, antibody detection, cross\u2010match resolution and workflow integration were assessed, alongside comparisons with dithiothreitol (DTT), papain, trypsin and DaraEx.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Daratumumab interference was neutralized in 99.5% of samples, with 86.2% resolved using the 10% protocol. Detection of 190\/197 RBC antibodies (96.4%) was preserved, including weak\/low\u2010titre antibodies. Neutralization was superior to DTT (93.3%), papain\/trypsin (84.9%) and DaraEx (68.4%; all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Titration studies confirmed efficacy across various ranges of daratumumab titres. The reagent integrated seamlessly into workflows, remained stable over time and avoided RBC modification.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Daratumumab interference was efficiently neutralized while preserving alloantibody detection. The robust performance and workflow compatibility provide a practical solution for pre\u2010transfusion testing in daratumumab\u2010treated patients, supporting safe and timely transfusions with reduced laboratory burden.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70269","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T02:21:09Z","timestamp":1776738069000},"page":"1065-1075","source":"Crossref","is-referenced-by-count":2,"title":["International multicentre evaluation of a new anti\u2010idiotypic anti\u2010daratumumab for resolving pre\u2010transfusion interferences"],"prefix":"10.1111","volume":"121","author":[{"given":"Arnaud","family":"Reggiani","sequence":"first","affiliation":[{"name":"Bio\u2010Rad Laboratories DiaMed GmbH  Cressier Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9609-3209","authenticated-orcid":false,"given":"Sofia Lejon","family":"Crottet","sequence":"additional","affiliation":[{"name":"Interregional Blood Transfusion SRC Ltd.  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Waldvogel","sequence":"additional","affiliation":[{"name":"Laboratoire d'immuno\u2010h\u00e9matologie transfusionnelle Hopitaux Universitaires de Gen\u00e8ve  Gen\u00e8ve Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Engstr\u00f6m","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service Zurich SRK  Schlieren Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serelina","family":"Coluzzi","sequence":"additional","affiliation":[{"name":"AOU Policlinico Umberto 1\u2010Sapienza UOC Immunoematologia e Medicina Trasfusionale  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Matteocci","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera San Camillo \u2013 Forlanini UOC Medicina Trasfusionale e Cellule Staminali  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis R.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"American Red Cross Michigan Region  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levent Tufan","family":"Kumas","sequence":"additional","affiliation":[{"name":"Ac\u0131badem Labmed  \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen M. K.","family":"de Vooght","sequence":"additional","affiliation":[{"name":"Central Diagnostics Laboratory UMC Utrecht  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2940-2604","authenticated-orcid":false,"given":"Jill R.","family":"Storry","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Transfusion Medicine Sk\u00e5ne University Hospital  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennylyn","family":"Flores","sequence":"additional","affiliation":[{"name":"Children's National Hospital Laboratory Blood Bank  Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00faria","family":"Nogu\u00e9s","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory Banc de Sang i Teixits  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2289-3420","authenticated-orcid":false,"given":"Carlos","family":"Cotorruelo","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunohematolog\u00eda Universidad Nacional de Rosario  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Rutherford","sequence":"additional","affiliation":[{"name":"Synnovis Analytics LLP, Guy's and St Thomas' Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eveline B.","family":"Nilsen","sequence":"additional","affiliation":[{"name":"The Blood Bank at Vestfold Hospital Trust  T\u00f8nsberg Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Pelc\u2010K\u0142opotowska","sequence":"additional","affiliation":[{"name":"Institute of Hematology and Transfusion Medicine  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Korzeniowska","sequence":"additional","affiliation":[{"name":"Regionalne Centrum Krwiodawstwa i Krwiolecznictwa w Lublinie  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Baffa","sequence":"additional","affiliation":[{"name":"S.C. Banca del Sangue e Immunoematologia  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna","family":"Sareneva","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Diagnostic Center Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nor Hafizah","family":"Ahmad","sequence":"additional","affiliation":[{"name":"National Blood Centre Immunohematology Reference Laboratory  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Szczudlo","sequence":"additional","affiliation":[{"name":"Regional Blood Donation and Blood Therapy Center  Katowice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athif","family":"Rahman","sequence":"additional","affiliation":[{"name":"Blood Transfusion Department Hammersmith Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laser","family":"Sanal","sequence":"additional","affiliation":[{"name":"Ankara Bilkent City Hospital Transfusion Center  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina Majewska","family":"Rochowiak","sequence":"additional","affiliation":[{"name":"Regionalne Centrum Krwiodawstwa i Krwiolecznictwa w Krakowie  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Salwierz","sequence":"additional","affiliation":[{"name":"Rzesz\u00f3w \u2013 Regionalne Centrum Krwiodawstwa i Krwiolecznictwa  Rzesz\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisaveta","family":"Grancharova","sequence":"additional","affiliation":[{"name":"Center of Transfusion Hematology, Military Medical Academy  Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bojana","family":"Zivkovic","sequence":"additional","affiliation":[{"name":"UKC Maribor  Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Alvarez","sequence":"additional","affiliation":[{"name":"UChicago Medicine Center for Care and Discovery  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareike","family":"Riedl","sequence":"additional","affiliation":[{"name":"Bio\u2010Rad AbD Serotec GmbH  Neuried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Knappik","sequence":"additional","affiliation":[{"name":"Bio\u2010Rad AbD Serotec GmbH  Neuried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.342.6165.1432"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10673"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3236"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2744"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-06-740944"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1506348"},{"key":"e_1_2_9_8_1","unstructured":"U.S. Food and Drug Administration.Daratumumab (DARZALEX) [Internet]. Available from:https:\/\/www.fda.gov\/drugs\/resources-information-approved-drugs\/daratumumab-darzalex. Last accessed 16 Jan 2026."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.21037\/aob-24-23"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2025017279"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.14341"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina60081247"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-327361"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13069"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/JBM.S213510"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02616"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14165754"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16687"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-08-833962"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13789"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-025"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12508"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2019-131345"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2450\/BloodTransfus.1137"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13150"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17006"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.08.012"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2007.070"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2013.07.025"},{"key":"e_1_2_9_30_1","volume-title":"AABB technical manual","author":"Cohn CS","year":"2020"},{"key":"e_1_2_9_31_1","first-page":"290","article-title":"New method for overcoming the interference produced by anti\u2010CD38 monoclonal antibodies in compatibility testing","volume":"18","author":"Izaguirre EC","year":"2020","journal-title":"Blood Transfus"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70269","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70269","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:28:51Z","timestamp":1783902531000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/vox.70269"],"URL":"https:\/\/doi.org\/10.1111\/vox.70269","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,20]]}}}