{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T02:28:55Z","timestamp":1786328935988,"version":"build-2736575974"},"reference-count":12,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>The human antiglobulin test or Coombs' test is a key tool in the diagnosis and classification of haemolytic anaemia and could present as falsely positive. A retrospective study has described that B\u2010type natriuretic peptide (NT\u2010proBNP), a biomarker used for heart failure (HF), could be an additional cause of false positives in the Coombs test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A prospective, observational, single\u2010centre study was conducted in adult patients diagnosed with HF between October 2024 and March 2025 at a tertiary hospital. The Coombs' test was considered as false positive in cases in which the specificity of the test included immunoglobulin G (IgG), the subsequent elution yielded a negative result, and blood haemolysis parameters were within normal ranges.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Thirty\u2010six patients were included, of whom nine (25%) had a false\u2010positive Coombs test. These patients had significantly higher levels of NT\u2010proBNP (10,825\u2009pg\/mL [2587\u201335,000] vs. 3314\u2009pg\/mL [278\u201311,856],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0076) and lower glomerular filtration rate (35.3\u2009mL\/min\/1.73\u2009m\n                      <jats:sup>2<\/jats:sup>\n                      [23.6\u201346.3] vs. 63.3\u2009mL\/min\/1.73\u2009m\n                      <jats:sup>2<\/jats:sup>\n                      [21.9\u201390.0],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0055) compared to patients with a negative Coombs test. No statistically significant associations were found with drugs used concomitantly for HF.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The results suggest that elevated NT\u2010proBNP could also be a cause of false positives in the Coombs test.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70284","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:34:36Z","timestamp":1778200476000},"page":"1245-1249","source":"Crossref","is-referenced-by-count":0,"title":["Association between\n                    <scp>NT\u2010proBNP<\/scp>\n                    and false\u2010positive Coombs test in patients with heart failure and elevated\n                    <scp>NT\u2010proBNP<\/scp>"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4642-6719","authenticated-orcid":false,"given":"Ana","family":"Lamilla \u00c1lvarez","sequence":"first","affiliation":[{"name":"Internal Medicine Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Asunci\u00f3n","family":"C\u00e1mara Rodenas","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sof\u00eda","family":"Russo Botero","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pau","family":"Aracil Boigues","sequence":"additional","affiliation":[{"name":"Cardiology Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"J\u00e1tiva S\u00e1ez","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Vidal Rico","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Santos Ca\u00f1amares","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma","family":"Rangel Algaba","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Ortiz\u2010Zuluaga","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefa Esperanza","family":"Marco Buades","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1402-4364","authenticated-orcid":false,"given":"Lorenzo","family":"F\u00e1cila Rubio","sequence":"additional","affiliation":[{"name":"Cardiology Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"P\u00e9rez\u2010Silvestre","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda de la Encarnaci\u00f3n","family":"L\u00f3pez\u2010Pav\u00eda","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department Valencia General University Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Manual pr\u00e1ctico de hematolog\u00eda cl\u00ednica","author":"Sanz MA","year":"2022"},{"key":"e_1_2_9_3_1","article-title":"Interference in the indirect antiglobulin test and direct antiglobulin test from rheumatoid factor","volume":"48","author":"Xu L","year":"2020","journal-title":"J Int Med 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