{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T02:28:56Z","timestamp":1786328936314,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201012\u20101\u20100441"],"award-info":[{"award-number":["W81XWH\u201012\u20101\u20100441"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["EDMS 5570"],"award-info":[{"award-number":["EDMS 5570"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Cold\u2010stored platelets (CSPs) can form aggregates, ultimately rendering them unusable. The causes of these aggregates and how to prevent them are poorly understood. This study aimed to identify potential factors associated with aggregate formation in CSPs stored in plasma for up to 14\u2009days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We obtained CSPs from 79 unique donors during a clinical trial. We retrospectively analysed aggregate rates among donors of different sexes, ages and blood groups. In a subgroup, samples were also tested by enzyme\u2010linked immunosorbent assay\u00a0for markers of thrombosis and inflammation. Platelets from mice lacking von Willebrand factor (VWF) and human apheresis platelets treated with VWF and caplacizumab were stored and assessed for platelet count and aggregate formation by visual inspection and flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Forty\u2010five (57%) units developed aggregates, and 34 (43%) did not. Units with aggregates had significantly lower soluble thrombomodulin levels and higher VWF levels approaching significance. In a multivariate analysis, platelets from female donors and donors with ABO type A were significantly more likely to form aggregates than those from male donors and donors with ABO type O, respectively. In the oldest donor cohort, units with aggregates were significantly less common than those without. Interfering with VWF levels and VWF function during storage did not reliably cause or prevent aggregates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Donor sex, age, ABO type and thrombomodulin were significantly associated with aggregate formation or prevention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70294","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T04:35:47Z","timestamp":1779683747000},"page":"1181-1191","source":"Crossref","is-referenced-by-count":0,"title":["Factors associated with aggregate formation in cold\u2010stored platelets"],"prefix":"10.1111","volume":"121","author":[{"given":"Samantha","family":"Huang","sequence":"first","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Mishima","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. Lawrence","family":"Bailey","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aastha","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Quesada","sequence":"additional","affiliation":[{"name":"Department of Manufacturing and Transfusion Services South Texas Blood and Tissue Center  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pat","family":"Klotz","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler","family":"Morrow","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Service Swedish Medical Center, Cherry Hill Campus  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Provencher","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Service Swedish Medical Center, Cherry Hill Campus  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6227-4088","authenticated-orcid":false,"given":"Junmei","family":"Chen","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"},{"name":"Department of Medicine, Division of Hematology and Oncology University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsung\u2010Lin","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Medical Services Bloodworks Northwest  Seattle Washington USA"},{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya","family":"Panch","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology and Oncology University of Washington  Seattle Washington USA"},{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"},{"name":"Department of Clinical Research Fred Hutchinson Cancer Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2866-1674","authenticated-orcid":false,"given":"Moritz","family":"Stolla","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest Research Institute  Seattle Washington USA"},{"name":"Department of Medicine, Division of Hematology and Oncology University of Washington  Seattle Washington USA"},{"name":"Department of Medical Services Bloodworks Northwest  Seattle Washington USA"},{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"},{"name":"Department of Clinical Research Fred Hutchinson Cancer Institute  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2025.7529"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13433"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196905152802004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2020.08.003"},{"key":"e_1_2_9_6_1","unstructured":"FDA guidance on alternative procedures for the manufacture of cold\u2010stored platelets intended for the treatment of active bleeding when conventional platelets are not available or their use is not practical. Available from:https:\/\/www.fda.gov\/regulatory\u2010information\/search\u2010fda\u2010guidance\u2010documents\/alternative\u2010procedures\u2010manufacture\u2010cold\u2010stored\u2010platelets\u2010intended\u2010treatment\u2010active\u2010bleeding\u2010when. 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