{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:18:58Z","timestamp":1789165138005,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Immunoglobulin A (IgA) deficiency occurs in approximately 1 in 14,000 individuals in Japan. Approximately 32% harbour anti\u2010IgA, most of which are naturally occurring and rarely cause anaphylaxis, although high\u2010titre anti\u2010IgA from alloimmunization can cause severe reactions. A stable supply of IgA\u2010deficient plasma is essential for minimizing this risk. We therefore developed a novel reagent for large\u2010scale identification of IgA\u2010deficient donors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Four mouse monoclonal anti\u2010IgA\u2010producing cell lines were established using the hybridoma method after immunizing mice with IgA protein. Purified antibodies were conjugated to carboxylate\u2010modified polystyrene latex beads for IgA measurement using the latex agglutination method on an automated analyser. Samples with low IgA concentrations were re\u2010tested by enzyme\u2010linked immunosorbent assay (ELISA) for confirmation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Adoption of a dual antibody assay design achieved a detection limit of 0.4\u2009mg\/dL, suitable for high\u2010throughput screening. Screening of 24,977 randomly selected donor samples between February 2022 and April 2023 yielded 4 low IgA cases. IgA deficiency was verified by supplemental ELISA, which was required solely for these four samples. Three of four had IgA levels &lt;0.05\u2009mg\/dL, which is the most common threshold for identifying IgA\u2010deficient donors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We have developed a new reagent for detecting IgA deficiency. The assay is automatable and appears useful for large\u2010scale screening of blood donors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70305","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T07:55:42Z","timestamp":1780386942000},"page":"1322-1329","source":"Crossref","is-referenced-by-count":0,"title":["A novel reagent for the screening of Immunoglobulin\n                    <scp>A<\/scp>\n                    \u2010deficient blood donors"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-257X","authenticated-orcid":false,"given":"Naoko","family":"Watanabe\u2010Okochi","sequence":"first","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Sato","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Okuyama","sequence":"additional","affiliation":[{"name":"Fujikura Kasei Co., Ltd.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Go","family":"Tomiyoshi","sequence":"additional","affiliation":[{"name":"Fujikura Kasei Co., Ltd.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Anazawa","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashige","family":"Shimoyamada","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Onodera","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Uchikawa","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeki","family":"Miyata","sequence":"additional","affiliation":[{"name":"Kanto\u2010Koshinetsu Block Blood Center, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antib8040057"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtauto.2019.100025"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1986.tb04851.x"},{"key":"e_1_2_9_5_1","first-page":"209","article-title":"IgA deficiency and blood transfusion management","volume":"41","author":"Shimada E","year":"2013","journal-title":"Test Technol"},{"key":"e_1_2_9_6_1","first-page":"579","article-title":"Identification and characterization of IgA deficiency and haptoglobin (Hp) deficiency among Japanese blood donors for the establishment of a registration system of plasma protein\u2010deficient donors","volume":"34","author":"Shimoyamada T","year":"2012","journal-title":"Ketsuekijigyo"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(68)90527-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196901232800404"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(05)80026-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3925\/jjtc.63.708"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02588.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-024-09007-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13543"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12796"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70305","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70305","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T21:58:50Z","timestamp":1789163930000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,2]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/vox.70305"],"URL":"https:\/\/doi.org\/10.1111\/vox.70305","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,2]]}}}