{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:18:57Z","timestamp":1789165137372,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007877","name":"Fujita Health University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007877","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Thawed plasma (TP) can be stored for several days to facilitate rapid emergency transfusion. However, in Japan, the 24\u2010h post\u2010thaw shelf life limit raises concerns about plasma wastage. We evaluated fibrinogen recovery in cryoprecipitate prepared from TP that was stored for 5\u2009days after thawing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Cryoprecipitate was prepared using the one\u2010step method (OSM,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912), two\u2010step method (TSM,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200915) and the TP stored for 5\u2009days post\u2010thaw (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200915). Cryoprecipitate was prepared using three protocols: OSM, in which fresh frozen plasma (FFP) was thawed once at 2\u20136\u00b0C for 24\u201330\u2009h; TSM, in which FFP was thawed at 2\u20136\u00b0C, refrozen at \u221230\u00b0C and then thawed again at 2\u20136\u00b0C for 24\u201330\u2009h; and TP, in which FFP was stored at 2\u20136\u00b0C for 5\u2009days after initial thawing, then refrozen and thawed as in the TSM. All samples were centrifuged after the final thawing to collect the cryoprecipitate. Fibrinogen recovery was calculated from fibrinogen concentrations, and bacteriological testing was performed on the cryoprecipitate prepared from TP.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Fibrinogen recovery differed significantly among the groups (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;\u20090.001), with the highest recovery in the TSM group, followed by the TP and OSM groups. Recovery in the TP group was significantly higher than that in the OSM group. No bacterial growth was detected in any of the samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Using stored TP to prepare cryoprecipitate could offer a more efficient way to manage resources while supporting emergency transfusion readiness.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70319","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T01:39:55Z","timestamp":1782178795000},"page":"1436-1439","source":"Crossref","is-referenced-by-count":0,"title":["Fibrinogen recovery in cryoprecipitate prepared from thawed plasma stored for 5\u2009days post\u2010thaw"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8180-1709","authenticated-orcid":false,"given":"Anna","family":"Yoshimine","sequence":"first","affiliation":[{"name":"Graduate School of Health Sciences Fujita Health University  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6307-3379","authenticated-orcid":false,"given":"Hideaki","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences Fujita Health University  Toyoake Japan"},{"name":"Department of Blood Transfusion Fujita Health University Hospital  Toyoake Japan"},{"name":"Department of Cellular and Molecular Biology Fujita Health University School of Medical Sciences  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuya","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Fujita Health University Hospital  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurina","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Fujita Health University Hospital  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Fujita Health University Hospital  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6748-4546","authenticated-orcid":false,"given":"Yohei","family":"Sakai","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences Fujita Health University  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Fujita Health University Hospital  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Doi","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology Fujita Health University School of Medical Sciences  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Fujita Health University Hospital  Toyoake Japan"},{"name":"Department of Cellular and Molecular Biology Fujita Health University School of Medical Sciences  Toyoake Japan"},{"name":"Department of Transfusion Medicine and Cell Therapy Fujita Health University School of Medicine  Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199502000-00006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ta.0000199432.88847.0c"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13156"},{"key":"e_1_2_8_5_1","volume-title":"Standards for blood banks and transfusion services","year":"2024"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13098"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.02063.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.70158"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3925\/jjtc.62.664"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/20.5.615"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3925\/jjtc.64.516"},{"key":"e_1_2_8_12_1","first-page":"114","article-title":"Effect of water bath versus refrigerator thaw on cryoprecipitate fibrinogen and factor VIII content using a pre\u2010pooled plasma experimental approach","volume":"52","author":"Swanson JA","year":"2024","journal-title":"Transfus Med 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