{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T16:32:20Z","timestamp":1786379540603,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Repeated blood donations deplete 200\u2013250\u2009mg of iron per donation. While biochemical consequences on iron stores are well documented, the role of circulating microRNAs (miRNAs) in maintaining iron homeostasis remains poorly characterized. This study investigated whether repeated blood donation alters iron metabolism\u2010related miRNA expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study compared 42 male participants: 21 repeat donors (donating three to four times annually; mean total donations: 15) and 21 first\u2010time donors. Plasma iron metabolism markers (ferritin, hepcidin, ferroportin, transferrin, soluble transferrin receptor 1 [sTfR1], soluble transferrin receptor 2 [sTfR2], unsaturated iron\u2010binding capacity [UIBC]) were quantified using enzyme\u2010linked immunosorbent assay (ELISA) and chemiluminescence methods. Expression of 13 miRNAs was analysed by quantitative reverse transcriptase polymerase chain reaction, normalized to U6 and quantified using the 2\n                      <jats:sup>\u2212\u0394\u0394Ct<\/jats:sup>\n                      method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Repeat donors exhibited significantly lower ferritin (median: 26.8 vs. 72.2\u2009ng\/mL;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), elevated red cell distribution width coefficient of variation (RDW\u2010CV) (13.86% vs. 13.07%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and reduced mean corpuscular haemoglobin concentration (MCHC) (33.80 vs. 34.23\u2009g\/dL;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.009) while maintaining normal haemoglobin. No significant differences were observed in any miRNA after multiple\u2010testing correction. However, miR\u2010130a showed a marginal decrease (fold change: 0.389;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0564; Cohen's\n                      <jats:italic>d<\/jats:italic>\n                      \u2009=\u20090.514), while miR\u2010200b and miR\u2010485\u20103p demonstrated non\u2010significant upward trends. Correlation analysis revealed distinct patterns between groups in iron regulatory protein relationships.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Repeated blood donations demonstrate \u2018compensated iron depletion\u2019. No significant differences were detected in the 13 selected iron\u2010homeostasis\u2010related miRNAs after multiple\u2010testing correction. Within the limits of this small, male\u2010only study evaluating a single miRNA panel, these findings indicate no detectable alteration, while underscoring the importance of ferritin monitoring; broader molecular pathways were not assessed, and given the limited statistical power, a Type II error cannot be excluded.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70344","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T16:20:48Z","timestamp":1786378848000},"source":"Crossref","is-referenced-by-count":0,"title":["Compensated iron depletion in repeated blood donations: Stability of circulating\n                    <scp>microRNAs<\/scp>\n                    despite reduced ferritin stores"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9408-5073","authenticated-orcid":false,"given":"Aziz","family":"Karaca","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Recep Tayyip Erdo\u011fan University  Rize Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6226-4712","authenticated-orcid":false,"given":"\u015eenol","family":"\u00c7itli","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Faculty of Medicine Recep Tayyip Erdo\u011fan University  Rize Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8321-4829","authenticated-orcid":false,"given":"Medeni","family":"Arpa","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Recep Tayyip Erdo\u011fan University  Rize Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1060-6503","authenticated-orcid":false,"given":"K\u00fcbra","family":"S\u00f6nmez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Recep Tayyip Erdo\u011fan University  Rize Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"51","article-title":"The regulation of iron absorption and homeostasis","volume":"37","author":"Wallace DF","year":"2016","journal-title":"Clin Biochem Rev"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03062.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu5072611"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1186\/s40001-023-01579-5","article-title":"Circulating microRNAs and hepcidin as predictors of iron homeostasis and anemia among school children: a biochemical and cross\u2010sectional survey analysis","volume":"28","author":"Al\u2010Rawaf HA","year":"2023","journal-title":"Eur J Med Res"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003408"},{"key":"e_1_2_9_7_1","article-title":"The crosstalk between microRNA and iron homeostasis","volume":"1","author":"Xu Z","year":"2014","journal-title":"Int J Genomic Med."},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12554"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3758\/BF03193146"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-85"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V50.3.441.441"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.2000.00239.x"},{"key":"e_1_2_9_15_1","first-page":"167","article-title":"The effect of repeated blood donations on the iron status of male Saudi blood donors","volume":"9","author":"Abdullah SM","year":"2011","journal-title":"Blood Transfus"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499768"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2021.103072"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12500"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12209"},{"key":"e_1_2_9_20_1","first-page":"37","article-title":"Prospective study on the effect of blood donation in the iron status","volume":"23","author":"Mantilla Gutierrez CY","year":"2021","journal-title":"Investig Andina"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12288-018-0954-9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-258467"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2019.232124"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22126493"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(03)00005-6"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.118521"},{"key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.3390\/ph18081104","article-title":"Iron deficiency and iron deficiency anemia: a comprehensive overview of established and emerging concepts","volume":"18","author":"Kolar\u0161 B","year":"2025","journal-title":"Pharmaceuticals (Basel)"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2996"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-06-857995"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(21)00168-X"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25706"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03401.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2010.03.004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.356717"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.08.015"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-016-2032-0"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44883"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.577387"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110481"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.020685"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-010-0849-4"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2006.12.002"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153200"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22729"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.10.002"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249713"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6062094"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2020.102523"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2013.11.005"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101613"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.09.015"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70344","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vox.70344","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vox.70344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T16:20:50Z","timestamp":1786378850000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vox.70344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":50,"alternative-id":["10.1111\/vox.70344"],"URL":"https:\/\/doi.org\/10.1111\/vox.70344","archive":["Portico"],"relation":{},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]},"article-number":"vox.70344"}}