{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:19:45Z","timestamp":1787624385398,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009033","name":"NHS Blood and Transplant","doi-asserted-by":"publisher","award":["159PATH25"],"award-info":[{"award-number":["159PATH25"]}],"id":[{"id":"10.13039\/100009033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009033","name":"NHS Blood and Transplant","doi-asserted-by":"publisher","award":["158PATH25"],"award-info":[{"award-number":["158PATH25"]}],"id":[{"id":"10.13039\/100009033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"abstract":"Abstract<\/jats:title>\n \n Background and Objectives<\/jats:title>\n Availability of new technologies has accelerated the pace of blood group discovery, with nine of 48 known systems being discovered in the past 5\u2009years alone, and yet uncharacterized antigens remain. This study involved investigations of samples from a female patient presenting with a moderate strength pan\u2010reactive antibody to a high\u2010prevalence antigen present on all red blood cells (RBCs) tested other than her own and her brother's.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n Samples underwent extensive serological testing; however, the antibody specificity could not be assigned to any known blood group antigens. Therefore, whole exome sequencing was performed alongside antigen expression studies to identify the gene variant responsible for this rare phenotype.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Both individuals were found to carry a homozygous variant c.644G>A (rs755819660; gnomAD allele frequency 0.0000044) in exon 6 of the\n F11R<\/jats:italic>\n gene, encoding junctional adhesion molecule\u2010A (JAM\u2010A). This variant encodes p.Arg215Gln located on the second extracellular immunoglobulin domain of JAM\u2010A. Expression of JAM\u2010A was demonstrated on all RBCs tested by flow cytometry, with cells of both the patient and her brother showing lower expression than control cells. Control (Arg215) and variant (Gln215) forms of JAM\u2010A were expressed as both soluble Fc fusion proteins and on Chinese hamster ovary cells. In both cases, mouse monoclonal anti\u2010JAM\u2010A recognized both forms, whilst the patient antibody only reacted with the Arg\u2010215\u2010JAM\u2010A control.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n These data demonstrate that JAM\u2010A carries a new high\u2010prevalence RBC antigen, lacking in the patient and her brother, thereby defining a novel blood group system encoded by\n F11R<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vox.70352","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:45:38Z","timestamp":1787622338000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification of a novel high\u2010prevalence red blood cell antigen on junctional adhesion molecule\u2010\n A<\/scp>\n defines a new blood group system"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3434-1973","authenticated-orcid":false,"given":"Tosti J.","family":"Mankelow","sequence":"first","affiliation":[{"name":"International Blood Group Reference Laboratory NHS Blood and Transplant Bristol UK"},{"name":"Component Development Laboratory NHS Blood and Transplant Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8244-4331","authenticated-orcid":false,"given":"Louise A.","family":"Tilley","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory NHS Blood and Transplant Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1412-9107","authenticated-orcid":false,"given":"Vanja","family":"Karamatic Crew","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory NHS Blood and Transplant Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Jones","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory NHS Blood and Transplant Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Izak","sequence":"additional","affiliation":[{"name":"Magen David Adom \u2013 National Blood Services Center Ramla Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liora","family":"Muncher","sequence":"additional","affiliation":[{"name":"Magen David Adom \u2013 National Blood Services Center Ramla Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vered","family":"Yahalom","sequence":"additional","affiliation":[{"name":"Blood Services and Apheresis Institute, Rabin Medical Center Petach Tiqva Israel"},{"name":"Gray Faculty of Medical and Health Sciences, Tel Aviv University Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8635-0997","authenticated-orcid":false,"given":"Nicole M.","family":"Thornton","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory NHS Blood and Transplant Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Red cell immunogenetics and blood group terminology ISBT working party. Available from:https:\/\/www.isbtweb.org\/isbt-working-parties\/rcibgt.html. Last accessed 19 May 2026."},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1182\/blood.2024026285","article-title":"The neutrophil antigen 3a\/b polymorphism in SLC44A2 unexpectedly encodes the Csa\/Csb red cell antigens","volume":"146","author":"Duval R","year":"2025","journal-title":"Blood"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1182\/blood.2024025099","article-title":"Deletions in the MAL gene result in loss of Mal protein, defining the rare inherited AnWj\u2010negative blood group phenotype","volume":"144","author":"Tilley LA","year":"2024","journal-title":"Blood"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1182\/blood.2022016504","article-title":"Missense mutations in PIEZO1, which encodes the Piezo1 mechanosensor protein, define Er red blood cell antigens","volume":"141","author":"Karamatic Crew V","year":"2023","journal-title":"Blood"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202216320","article-title":"Lack of the human choline transporter\u2010like protein SLC44A2 causes hearing impairment and a rare red blood phenotype","volume":"15","author":"Koehl B","year":"2023","journal-title":"EMBO Mol Med"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1038\/s41467-020-17060-4","article-title":"Disruption of the tumour\u2010associated EMP3 enhances erythroid proliferation and causes the MAM\u2010negative phenotype","volume":"11","author":"Thornton N","year":"2020","journal-title":"Nat Commun"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.gene.2005.08.025","article-title":"Genomic structure, organization and promoter analysis of the human F11R\/F11 receptor\/junctional adhesion molecule\u20101\/JAM\u2010A","volume":"366","author":"Sobocki 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polarity","volume":"116","author":"Ebnet K","year":"2003","journal-title":"J Cell Sci"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1093\/emboj\/20.16.4391","article-title":"X\u2010ray structure of junctional adhesion molecule: structural basis for homophilic adhesion via a novel dimerization motif","volume":"20","author":"Kostrewa D","year":"2001","journal-title":"EMBO J"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1091\/mbc.e14-01-0018","article-title":"Trans\u2010dimerization of JAM\u2010A regulates Rap2 and is mediated by a domain that is distinct from the cis\u2010dimerization interface","volume":"25","author":"Monteiro AC","year":"2014","journal-title":"Mol Biol Cell"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1007\/s00018-017-2729-0","article-title":"Junctional adhesion molecule\u2010A: functional diversity through molecular promiscuity","volume":"75","author":"Steinbacher T","year":"2018","journal-title":"Cell Mol Life Sci"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1529\/biophysj.108.135491","article-title":"LFA\u20101 binding destabilizes the JAM\u2010A homophilic interaction during leukocyte transmigration","volume":"96","author":"Wojcikiewicz EP","year":"2009","journal-title":"Biophys J"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/ni755","article-title":"JAM\u20101 is a ligand of the beta(2) integrin LFA\u20101 involved in transendothelial migration of leukocytes","volume":"3","author":"Ostermann G","year":"2002","journal-title":"Nat Immunol"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"6259","DOI":"10.4049\/jimmunol.173.10.6259","article-title":"The functional interaction of the beta 2 integrin lymphocyte function\u2010associated antigen\u20101 with junctional adhesion molecule\u2010A is mediated by the I domain","volume":"173","author":"Fraemohs L","year":"2004","journal-title":"J Immunol"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1091\/mbc.e12-06-0481","article-title":"Tetraspanin CD9 links junctional adhesion molecule\u2010A to \u03b1v\u03b23 integrin to mediate basic fibroblast growth factor\u2010specific angiogenic signaling","volume":"24","author":"Peddibhotla SS","year":"2013","journal-title":"Mol Biol Cell"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1182\/blood-2003-04-1114","article-title":"Signaling through JAM\u20101 and alphavbeta3 is required for the angiogenic action of bFGF: dissociation of the JAM\u20101 and alphavbeta3 complex","volume":"102","author":"Naik MU","year":"2003","journal-title":"Blood"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/s00018-022-04140-5","article-title":"JAM\u2010A interacts with \u03b13\u03b21 integrin and tetraspanins 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